A method for molecularly imprinted SPE of the melamine from environmental water samples was investigated. Cyromazine-imprinted polymers were synthesized in water-methanol systems for the selective extraction of melamine from aqueous samples, followed by HPLC analysis. Molecular recognition properties and binding capability to melamine were evaluated by adsorption test and Scatchard analysis, which showed the dissociation constant (KD) and the maximum binding quantity (Qmax) were 0.032 micromol/L and 4.77 micromol/g for high affinity binding site, and 0.26 micromol/L and 19.10 micromol/g for lower affinity binding site, respectively. Under the optimum extraction protocol, the method can be successfully applied to selectively extract and enrich melamine in environmental water. The linearity was ranged from 0.500 to 100.0 ng/mL (r > 0.999) in tap water, lake water, and seawater analysis. When 50 mL of the water samples loaded, the LODs of the method were ca. 0.1 ng/mL, and the LOQs were ca. 0.5 ng/mL. The mean recoveries of melamine from blank water samples spiked at 0.5, 5.0, and 50 ng/mL were more than 86.3%, with the RSD less than 8.8%.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.