Background Previous studies have shown that feeding a high‐energy formula (HF) to infants after cardiac surgery increases energy intake, with fewer side effects on cardiopulmonary function. However, impacts on weight gain and gastrointestinal function remain unclear. Aims and objectives To determine the impact of HF compared with standard formula on weight gain and gastrointestinal tolerance in postoperative infants with congenital heart disease. Design This was a randomized controlled trial. Methods The setting of the study was at a 20‐bed cardiac intensive care unit at a tertiary children's hospital in China. Study population included infants <1 year of age who underwent cardiac surgery and were allocated to the intervention group (n = 32) or control group (n = 32). The intervention group received HF (100 kcal/100 mL), and the control group received standard formula (67 kcal/100 mL) for 7 days during the stabilized postoperative period at the cardiac intensive care unit. Primary outcomes were weight gain and gastrointestinal intolerance. Secondary outcomes were energy intake and standard intensive care characteristics. Results Infants who received HF (n = 30) showed less weight loss than those who received standard formula (n = 29); −16 g [95% confidence interval (CI): −74 to 42] versus −181 g (95% CI: −264 to −99), P = 0·001. The evaluation of gastrointestinal intolerance showed that the intervention group had several side effects, such as abdominal distension (n = 1), gastric retention (n = 2) and diarrhoea (n = 1), while the control group had no problems. Enteral energy intake in the intervention group was higher than the control group from day three. Conclusion Infants after cardiac surgery fed with HF gained more weight but had increased feeding intolerance. However, the feeding intolerance symptoms could be relieved by medication and did not affect feeding advancement. Relevance to clinical practice Paediatric intensive care clinicians should consider gradually increasing the energy density of the formula during feeding and assess feeding intolerance signs in some children with malnutrition after cardiac surgery.

Objectives microRNA‐29 (miR‐29) family have shown different expression patterns in cardiovascular diseases. Our study aims to explore the effect and mechanism of miR‐29 family on cardiac development. Materials and methods A total of 13 patients with congenital heart disease (CHD) and 7 controls were included in our study. Tissues were obtained from the right ventricular outflow tract (RVOT) after surgical resection or autopsy. The next‐generation sequencing was applied to screen the microRNA expression profiles of CHD. Quantitative RT‐PCR and Western blot were employed to measure genes expression. Tg Cmlc2: GFP reporter zebrafish embryos were injected with microRNA (miRNA) to explore its role in cardiac development in vivo. Dual‐luciferase reporter assay was designed to validate the target gene of miRNAs. CCK‐8 and EdU incorporation assays were performed to evaluate cardiomyocyte proliferation. Results Our study showed miR‐29b‐3p expression was significantly increased in the RVOT of the CHD patients. Injection of miR‐29b‐3p into zebrafish embryos induced higher mortality and malformation rates, developmental delay, cardiac malformation and dysfunction. miR‐29b‐3p inhibited cardiomyocyte proliferation, and its inhibitor promoted cardiomyocyte proliferation in vitro and in vivo. Furthermore, we identified that miR‐29b‐3p influenced cardiomyocyte proliferation by targeting NOTCH2, which was down‐regulated in the RVOT of the CHD patients. Conclusion This study reveals that miR‐29b‐3p functions as a novel regulator of cardiac development and inhibits cardiomyocyte proliferation via NOTCH2, which provides novel insights into the aetiology and potential treatment of CHD.

Objective: To review the evolution of the management approach for pulmonary atresia with intact ventricular septum (PAIVS) in the past two decades and to assess its impact on patient outcomes. Design and patients: Retrospective review of the management and outcomes of 94 patients (55 male patients) with PAIVS diagnosed between July 1980 and August 2003. Settings: Tertiary paediatric cardiac centre. Results: Seven patients died before interventions. Of the remaining 87 patients who underwent intervention at a median age of 9 days (from 1 day to 2 years), 12 had right ventricular outflow tract reconstruction (RVOTR), 42 had closed pulmonary valvotomy (CPV), and 15 had laser assisted valvotomy with balloon valvoplasty. A systemic-pulmonary shunt was inserted in 18 patients, six of whom had subsequent RVOTR (n = 4) or laser assisted valvotomy (n = 2). Since 1990, catheter intervention accounted for 38% (17 of 45) of the right ventricular outflow procedures. The mean (SEM) freedom from reintervention was 93 (7)%, 71 (12)%, and 57 (13)% after RVOTR, 75 (7)%, 40 (8)%, and 14 (6)% after CPV, and 54 (13)%, 24 (12)%, and 16 (10)% after laser assisted valvotomy at one month, six months, and one year, respectively (RVOTR versus CPV, p , 0.001; RVOTR versus laser assisted valvotomy, p = 0.001). Low cardiac output syndrome was significantly less common after catheter intervention than after RVOTR (0% v 44%, p = 0.003) or CPV (0% v 29%, p = 0.01). The overall mean (SEM) survival was 77 (5)% and 70 (5)% at one and five years, respectively, and the overall mortality was 33% (29 of 87). There were no significant differences in survival between the three groups. Conclusions: Multiple interventions are often required in the treatment algorithm of PAIVS. The shift towards increased use of the transcatheter approach has reduced the occurrence of postprocedural low cardiac output syndrome.I n pulmonary atresia with intact ventricular septum (PAIVS), morphological diversity of the right ventricle (RV) and coronary arterial anatomy necessitates different management strategies.1 2 Whereas univentricular repair and heart transplantation are options for patients with a diminutive RV or an RV dependent coronary circulation, 2 3 one and a half and biventricular repairs are procedures of choice for those with varying degrees of RV hypoplasia. [2][3][4] In the case of RV hypoplasia, early establishment of continuity between the RV and pulmonary trunk is ideal, as it may facilitate a definitive biventricular repair by promoting RV growth. 5-8Surgical procedures including RV outflow tract reconstruction (RVOTR) 4 9 and pulmonary valvotomy 10 11 have been used to relieve the outflow tract obstruction. In the past decade, transcatheter laser 12 or radiofrequency assisted 13 pulmonary valvotomy has been added to the armamentarium for the management of PAIVS. In selected patients with a well developed tripartite RV, the radiofrequency assisted valvotomy procedure has been shown to be more efficacious in decompressing the RV and associated with lower...

We aimed to evaluate the outcome and regional and global left-ventricular (LV) function after aortic valve repair in children with congenital aortic valve disease. Thirty-two consecutive patients with a mean age of 12.62 years (4 months to 18 years) undergoing aortic valve repair due to valve stenosis (AS group, n = 21) or aortic regurgitation (AR group, n = 11) were studied during a follow-up period of 12 months regarding change and adaptation of myocardial function using conventional and novel echocardiographic methods, including two-dimensional (2D) strain echocardiogram. Conventional and 2D strain echocardiographic studies were performed and analyzed off-line using commercially available software (EchoPac 6.1.0, GE). Peak aortic valve gradient decreased from 62.04 ± 30.34 mmHg before surgery to 22.80 ± 14.13 mmHg 2 weeks after surgery and to 35.73 ± 22.11 mmHg 12 months after surgery (p = 0.01). The degree of AR decreased significantly to grade 0 in 20 children and to grade I in 12. There was a significant decrease of thickness of the interventricular septum (IVS) and posterior wall resulting in improvement of LV mass index (p = 0.007, p = 0.043, and p = 0.001, respectively). Significant decrease of myocardial thickness was found, especially in the IVS, in the AS group (p = 0.008), and a significant decrease in LV end-diastolic dimension (EDD) was found in the AR group (p = 0.007). 2D strain analysis showed that global peak strain, global systolic strain rate, and global early diastolic strain rates improved significantly for all patients during the study period after aortic valve repair (p < 0.001, p = 0.037, and p = 0.018, respectively). The global strain and strain rates correlated significantly to IVS thickness (r = 0.002 and r = 0.003, respectively), LV mass index (r = 0.02 and r = 0.015, respectively), and EDD (r = 0.26 and r = 0.005, respectively). Aortic valve repair surgery in pediatric patients results in improvement of global and regional systolic and diastolic LV parameters, which was better shown by 2D strain parameters rather than conventional echocardiographic parameters.

1The independent reproduction of research results is a cornerstone of experimental research, yet it is 2 beset by numerous challenges, including the quality and veracity of reagents and materials. Much of 3 life science research depends on life materials, including human tissue culture cells. In this study we 4 aimed at determining the degree of variability in the molecular makeup and the ensuing phenotypic 5 consequences in commonly used human tissue culture cells. We collected 14 stock HeLa aliquots 6 from 13 different laboratories across the globe, cultured them in uniform conditions and profiled the 7 genome-wide copy numbers, mRNAs, proteins and protein turnover rates via genomic techniques 8 and SWATH mass spectrometry, respectively. We also phenotyped each cell line with respect to the 9 ability of transfected Let7 mimics to modulate Salmonella infection. 10 11We discovered significant heterogeneity between HeLa variants, especially between lines of the 12 CCL2 and Kyoto variety. We also observed progressive divergence within a specific cell line over 13 50 successive passages. From the aggregate multi-omic datasets we quantified the response of the 14 cells to genomic variability across the transcriptome and proteome. We discovered organelle-specific 15 proteome remodeling and buffering of protein abundance by protein complex stoichiometry, 16 mediated by the adaptation of protein turnover rates. By associating quantitative proteotype and 17 phenotype measurements we identified protein patterns that explained the varying response of the 18 different cell lines to Salmonella infection. 19 20Altogether the results indicate a striking degree of genomic variability, the rapid evolution of 21 genomic variability in culture and its complex translation into distinctive expressed molecular and 22 phenotypic patterns. The results have broad implications for the interpretation and reproducibility of 23 research results obtained from HeLa cells and provide important basis for a general discussion of the 24 value and requirements for communicating research results obtained from human tissue culture cells. 25 7 molecular analyses, we centrally cultured all 14 cell lines for an additional three passages in our 126 group. To minimize experimental variability, the same researcher cultured the cells using the same 127 culture media and protocol. 128 129As a starting point for the evaluation of HeLa cell heterogeneity, we measured gene copy number 130 variation (CNV) by array-CGH (aCGH). Previous studies reported genome instability within and 131 between different HeLa strains 18, 25-28 . To investigate and display the ploidy variation of the strains 132 studied we projected the CNV data onto the reference human genomic map (Figure 1b). We 133 discovered pervasive CNV differences organized by domains, large chromosomal segments, and 134 even whole chromosomes. Particularly notable were ploidy changes at Chromosomes (Chr) 1, 2, 6, 135 9, 10, 17, 19, 21, 22, and X. (Figure 1b). On average, the HeLa genome of all cells tested...

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Doppler and tissue Doppler-derived E/e' ratio is related to simultaneous invasive measurement of EDP in a heterogeneous group of patients with CHD and may provide an additional surrogate non-invasive estimation of ventricular diastolic performance in the routine follow-up of these patients.

Single-layered, double-layered, and triple-layered Pt nanoparticles with a well-defined arrangement were encapsulated inside metal–organic frameworks (MOFs) to investigate the catalytic performance influenced by the progressive increasing of Pt nanoparticles inside MOFs; the results show that the catalytic activity of the Pt–MOF hybrid catalysts increases progressively with the progressive increasing of the Pt nanoparticles inside MOFs. Progressive increasing of Pt nanoparticles with a multiple-layered manner inside MOFs provides a new route for designing well-organized hybrid catalysts of noble metal nanoparticles and MOFs with enhanced catalytic activity.