Atorvastatin (ATV) may support mesenchymal stem cells (MSC) survival in post-infarct myocardium (MI) as inflammatory reactions, oxidative stress and hypoxia condition get started in such tissues after damage. However, limited aqueous insolubility and rapid first-pass metabolism reduce the systemic availability of ATV. The aim of the present investigation was to develop ATV loaded nanoparticles (ATVNPs) which might ensure the maximum availability of ATV in systemic circulation for longer duration and to strengthen the support to MSC survival. ATVNPs were synthesized using double emulsion solvent evaporation method and characterized as spherical shape, positive charged, nanoparticles of uniform size distribution and higher entrapment efficiency. ATVNPs were non-cytotoxic and showed sustained release (up to 28 days). Assessment of cardiac function (in terms of echocardiographic and left heart catheterization parameters) and cytokines estimation revealed efficient improvement in post-infarct myocardium condition of rat. In conclusion, ATVNP was developed successfully that may ensure safe, cost effective, and efficacious treatment of post-infarct myo-cardium when compared with that of MSC alone and MSC supplemented with ATV solution.
Compared with clopidogrel, ticagrelor can achieve better n antiplatelet effect for patients with acute myocardial infarction undergoing percutaneous coronary intervention (PCI). It can effectively reduce the incidence of postoperative adverse cardiac and cerebrovascular events and control the rate of adverse reactions within the acceptable range.
assessed while the plasma level of VPO1 in patients and the expression of VPO1 in arterial tissues was measured. Cultured human aorta vascular smooth muscle cells were treated with ANGII, and the proliferation activity, VPO1 expression, H 2 O 2 and HOCL level were examined. The effect of VPO1 RNA interference, apocynin, catalase and PD98059 on VPO1 expression and the proliferation activity of cells were observed. Results The VPO1 level/expression was significantly increased in patients with essential hypertension and in spontaneously hypertensive rats concomitant with definite vascular remolding by evaluating the intima-media thickness, pressure-strain elastic modulus and stiffness index of carotid artery in patients, as well as the media thickness, lumen diameter, media thickness/lumen diameter ratio and mean nuclear area in artery media in spontaneously hypertensive rats. The angiotension II-stimulated cell proliferation of human aorta smooth muscle cells was inhibited by knockdown of VPO1 using small hairpin RNA. Moreover, the NADPH oxidase inhibitor, apocynin, the hydrogen peroxide scavenger, catalase, but not the ERK1/2 inhibitor, PD98059 attenuated Ang II-mediated upregulation of VPO1 and generation of hypochlorous acid. Conclusions VPO1 is a novel regulator of vascular smooth muscle cell proliferation via NADPH oxidase/H 2 O 2 /VPO1/ERK1/2 pathways and plays an important role in vascular remodelling during hypertension. Background and Objects Elevated blood pressure causes a change in vascular remodelling and arterial stiffeness. Dynamic development of the inflammatory reaction may play a role in the early increase of blood pressure. Monocyte chemoattractant protein-1 (MCP-1) which has a chemotactic effect on monocytes/macrophages, is an initial factor of inflammation. However, whether monocyte chemoattractant protein-1 (MCP-1) is altered in the change of large arterial structure and function in prehypertensive subjects has been incompletely investigated. e0595 ANGIOTENSINII MODULATES ION PUMPS OF SMOOTH MUSCLE CELLS DERIVED FROM UMBILICAL ARTERY OF HUMAN NEONATES WITH HYPERTENSIVE FAMILY HISTORYMethod According to the criteria of JNC7, 160 subjects were divided into three groups: (1) normotensive group (n¼57), (2) prehypertensive group (n¼50) and (3) hypertensive group (n¼53). Brachium-ankle pulse wave velocity (BaPWV) was measured by an automatic waveform analyser (Form PWV/ABI) and carotid artery intima-media thickness (IMT) was determined ultrasonographically. MCP-1 mRNA level were obtained by real time RT-PCR.Result In prehypertensives, MCP-1, baPWV and IMT levels are higher than that in normotensives (p<0.01) and lower than that in hypertensives (p<0.01). MCP-1 mRNA level correlated linearly and significantly with baPWV and IMT (p<0.01), even after adjustments for confounding variables.Conclusions Large artery remodelling has been found in prehypertensive subjects. PWV and IMTwere closely related to the level of blood pressure. MCP-1 may play a role structural and functional vascular changes in preh...
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