BACKGROUND: Early diagnosis of tuberculosis (TB) cases in limited resource remains challenging. It is urgent to identify the new diagnostic tools which can control the spread of disease with accurate and rapid test. AIM: This study aimed to investigate the levels of infection markers: Composite bacterial infection index (CBII) and serum amyloid A (SAA) protein in pulmonary TB (PTB), and their healthy household contacts, as the alternative diagnostic markers for TB. METHODS: CBII and SAA were measured from 44 new PTB patients, and 31 household contact serum samples. The value of CBII was calculated from neutrophils, lymphocytes, monocytes, erythrocyte sedimentation rate, and high-sensitivity C-reactive protein (hs-CRP) level. hs-CRP and SAA levels were quantified from their serum samples using ELISA. QuantiFERON-TB Gold Plus (interferon gamma release assay [IGRA]) was used to screen latent TB infection among household contacts. RESULTS: Among 31 household contacts, there were 24 positive IGRA results and the rest (n = 7) had negative results. PTB patients exhibited significantly higher level CBII in the serum specimens, than those in household contact (p < 0.0001). There was no significant difference in the SAA level between TB cases and household contacts (p = 0.679). CONCLUSIONS: CBII can be used as one of the biomarkers for the identification of PTB from the serum specimens.
Latar Belakang: Kondiloma Akuminata (KA) atau venereal warts adalah infeksi menular seksual (IMS) disebabkan oleh Human Papilloma Virus (HPV) berbentuk papilomatosis, permukaan verukosa mengenai kulit dan mukosa. Lesi terdapat didaerah kelamin dan atau anus. Infeksi oleh HPV adalah IMS paling umum di seluruh dunia, mengenai 9 hingga 13 persen dari populasi umum yang terinfeksi. Di Indonesia, angka kejadian KA bervariasi, tetapi lebih banyak menunjukkan KA sebagai angka paling tinggi diantara IMS lain. Infeksi Menular Seksual dapat meningkatkan risiko terjadinya penyebaran infeksi oleh HIV, dan infeksi HIV juga memungkinkan peningkatan prevalensi terjadinya IMS. Infeksi HIV menjadi faktor resiko terjadinya penularan KA. Faktor risiko lain termasuk riwayat kontak seksual berganti pasangan, kontak seksual dengan sesama jenis, dan infeksi menular seksual lainnya. Hasil: Dilaporkan pasien laki-laki 39 tahun, pekerja swasta, belum menikah, dengan keluhan benjolan kecil diperianal, kadang gatal, dialami sebulan sebelumnya. Pasien terkonfirmasi HIV dan mengkonsumsi obat Anti Retro Viral (ARV) 3 bulan sebelumnya. Pemeriksaan fisik ditemukan papul dan nodul, permukaan verukosa, batas tegas, konsistensi padat. Uji acetowhite menunjukan hasil positif. Pernah dilakukan terapi tinktura podofillin, tetapi tidak memberikan perbaikan. Dilakukan terapi bedah laser CO2 dan didapatkan respons yang baik. Kesimpulan: Salah satu modalitas terapi yang aman dan memberikan respon perbaikan cepat bagi penderita KA dan HIV adalah dengan laser CO2
Natural resistance-associated macrophage protein 1 (NRAMP1) gene is regarded to play an important role in human vulnerability against various infections, including mycobacterial infection. This research purpose was to investigate the correlation between NRAMP1 gene expressions and protein level with susceptibility to tuberculosis (TB) infection and their contacts. NRAMP1 gene expression and protein level from 30 active TB patients, 27 latent TB infection household contacts, and 33 healthy subjects as control were examined by the real-time polymerase chain reaction and enzyme-linked immunosorbent assay method. NRAMP1 gene expression value in the healthy group was 4.61 times higher than active TB patients (p = 0.025) and the NRAMP1 expression value in the healthy group was 4.02 times higher than in the latent TB group (p = 0.117). The mean protein level of NRAMP1 was 196.41, 199.27, and 224.70 ng/ ml in the active TB, latent TB, and healthy groups, respectively (p > 0.05). NRAMP1 expression was downregulated and protein level was lower in active TB patients than latent TB patients and healthy individuals.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.