Background
Radioprotectors safeguard biological system exposed to ionizing radiation (IR) by protecting normal cells from radiation damage during radiotherapy. Due to the toxicity and limited clinical utility of the present radioprotectors, it prompts us to identify novel radioprotectors that could alleviate IR-induced cytotoxicity of normal tissues.
Aims and Methods
To identify new radioprotectors, we screened a chemical molecular library comprising 253 compounds in normal human fibroblasts (HFs) or 16HBE cells upon IR by CCK-8 assays and clonogenic survival assays. Fasudil was identified as a potential effective radioprotector.
Results
The results indicated that Fasudil exerts radioprotective effects on HFs against IR-induced DNA double-strand breaks (DSBs) through the regulation of DSB repair. Fasudil increased homologous recombination (HR) repair by 45.24% and decreased non-homologous end-joining (NHEJ) by 63.88% compared with untreated cells, without affecting changes to cell cycle profile. We further found that fasudil significantly facilitated the expression and foci formation of HR core proteins such as Rad51 and BRCA1 upon IR, and decreased the expression of NHEJ-associated proteins such as DNA-PKcs at 24 h post-IR.
Conclusion
Our study identified fasudil as a novel radioprotector that exert radioprotective effects on normal cells through regulation of DSB repair by promoting HR repair.
How to achieve the personalized three-dimensional human modeling and simulation is based on the extraction of the skin contour and data. In this paper, as the characteristics of the medical images, a simple processing simulation for the medical images is provided. Firstly, the human body slice images should be preprocessed and filtered. Secondly, the contour of processed image should be tracked. Finally, according to achieved results, the data of the two-dimensional contour can be extracted and a complete data between the skin and the skeleton can be acquired. The results achieved from this paper can help designers find the right methods to analysis the slice images, which providing acceptable data for the threedimensional reconstruction.
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