Due to its extreme salinity and high Mg concentration the Dead Sea is characterized by a very low density of cells most of which are Archaea. We discovered several underwater fresh to brackish water springs in the Dead Sea harboring dense microbial communities. We provide the first characterization of these communities, discuss their possible origin, hydrochemical environment, energetic resources and the putative biogeochemical pathways they are mediating. Pyrosequencing of the 16S rRNA gene and community fingerprinting methods showed that the spring community originates from the Dead Sea sediments and not from the aquifer. Furthermore, it suggested that there is a dense Archaeal community in the shoreline pore water of the lake. Sequences of bacterial sulfate reducers, nitrifiers iron oxidizers and iron reducers were identified as well. Analysis of white and green biofilms suggested that sulfide oxidation through chemolitotrophy and phototrophy is highly significant. Hyperspectral analysis showed a tight association between abundant green sulfur bacteria and cyanobacteria in the green biofilms. Together, our findings show that the Dead Sea floor harbors diverse microbial communities, part of which is not known from other hypersaline environments. Analysis of the water’s chemistry shows evidence of microbial activity along the path and suggests that the springs supply nitrogen, phosphorus and organic matter to the microbial communities in the Dead Sea. The underwater springs are a newly recognized water source for the Dead Sea. Their input of microorganisms and nutrients needs to be considered in the assessment of possible impact of dilution events of the lake surface waters, such as those that will occur in the future due to the intended establishment of the Red Sea−Dead Sea water conduit.

The Dead Sea is a closed lake, the water level of which is lowering at an alarming rate of about 1 m/year. Factors difficult to determine in its water balance are evaporation and groundwater inflow, some of which emanate as submarine groundwater discharge. A vertical buoyant jet generated by the difference in densities between the groundwater and the Dead Sea brine forms at submarine spring outlets. To characterize this flow field and to determine its volumetric discharge, a system was developed to measure the velocity and density of the ascending submarine groundwater across the center of the stream along several horizontal sections and equidistant depths while divers sampled the spring. This was also undertaken on an artificial submarine spring with a known discharge to determine the quality of the measurements and the accuracy of the method. The underwater widening of the flow is linear and independent of the volumetric spring discharge. The temperature of the Dead Sea brine at lower layers primarily determines the temperature of the surface of the upwelling, produced above the jet flow, as the origin of the main mass of water in the submarine jet flow is Dead Sea brine. Based on the measurements, a model is presented to evaluate the distribution of velocity and solute density in the flow field of an emanating buoyant jet. This model allows the calculation of the volumetric submarine discharge, merely requiring either the maximum flow velocity or the minimal density at a given depth.