Background and AimsTo evaluate the effect of intraparenchymal transplantation of mesenchymal bone marrow-derived stem cells (BMSCs) in patients with hepatitis C virus (HCV)-related liver cirrhosis (LC).MethodsMononuclear cells were isolated from patient bone marrow and were passaged several times in vitro in order to reach the required volume. Attributes of the BMSCs were evaluated by the presence of the surface markers CD105+, CD90+, and CD73+. Cells from each passage were evaluated for sterility, and they were transplanted intraparenchymally into liver tissue. Clinical and laboratory data were evaluated and morphological studies of liver biopsy were performed prior to and 6 months after transplantation.ResultsOn clinical evaluation, the general state of these patients was improved at 1 month following transplantation of BMSCs. At 1 and 6 months post-transplantation, jaundice was absent in four (67%) patients. After 6 months, functional hepatic indices were improved, i.e. decrease of ALT and AST activity and bilirubin level. However, these decreases were not statistically different (P>0.05). Expression of CD34 and α-SMA in liver biopsy samples were decreased at 6 months after transplantation, consistent with structural improvements in mitochondria and nuclear compartments.ConclusionsIntraparenchymal transplantation of autologous BMSCs improved the functional condition of the liver, stimulated reparative processes in hepatocytes, and decreased extracellular matrix protein (EMP) count in hepatic tissues of patients with LC. It was well tolerated and was not associated with any complications both during and after BMSC transplantation.
The authors studied the influence of the combination of TGF? and IGF growth factors, as well as the differentiation time, on the induction of MSC chondrogenesis in vitro. It is proved that MSCs located in 2D and 3D systems, when exposed to TGF?/ IGF, showed the signs of early chondroblast-like cells in 7 days. The TGF?/ IGF used for the induction of MSCs is more preferred, because it results in a more pronounced hypertrophic-suppression effect. The absence of significant differences in gene expression (excepting Sox9) on the 7th and 21st days of chondrogenic differentiation allows the process to be reduced in vitro to 7 days.
The ability of mesenchymal stem cells (MSCs) isolated from the decidua, chorionic tissue and amniotic membrane of the placenta to the self-renewal and the proliferation was investigated. Our results revealed that the number of colony forming unit-fibroblast (CFU-F) and the growth rate of MSCs were higher in the decidua and chorionic tissue compared to the amniotic membrane. Decidua MSCs and chorionic MSCs possessed a similar powerful proliferative potential and increased in 1010- fold in cultures for 3 months, that is 103 times more then the amniotic MSCs. The cumulative population doubling (PD) of placenta-derived MSCs was significantly higher at all passages then PD of bone marrow derived MSCs. The decidua and chorionic tissue of the placenta are ideal MSCs sources for cell based therapy.
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