Conspectus
Despite the astonishing diversity of naturally
occurring biocatalytic
processes, enzymes do not catalyze many of the transformations favored
by synthetic chemists. Either nature does not care about the specific
products, or if she does, she has adopted a different synthetic strategy.
In many cases, the appropriate reagents used by synthetic chemists
are not readily accessible to biological systems. Here, we discuss
our efforts to expand the catalytic repertoire of enzymes to encompass
powerful reactions previously known only in small-molecule catalysis:
formation and transfer of reactive carbene and nitrene intermediates
leading to a broad range of products, including products with bonds
not known in biology. In light of the structural similarity of iron
carbene (FeC(R1)(R2)) and iron nitrene
(FeNR) to the iron oxo (FeO) intermediate involved
in cytochrome P450-catalyzed oxidation, we have used synthetic carbene
and nitrene precursors that biological systems have not encountered
and repurposed P450s to catalyze reactions that are not known in the
natural world. The resulting protein catalysts are fully genetically
encoded and function in intact microbial cells or cell-free lysates,
where their performance can be improved and optimized by directed
evolution. By leveraging the catalytic promiscuity of P450 enzymes,
we evolved a range of carbene and nitrene transferases exhibiting
excellent activity toward these new-to-nature reactions. Since our
initial report in 2012, a number of other heme proteins including
myoglobins, protoglobins, and cytochromes c have
also been found and engineered to promote unnatural carbene and nitrene
transfer. Due to the altered active-site environments, these heme
proteins often displayed complementary activities and selectivities
to P450s.
Using wild-type and engineered heme proteins, we and
others have
described a range of selective carbene transfer reactions, including
cyclopropanation, cyclopropenation, Si–H insertion, B–H
insertion, and C–H insertion. Similarly, a variety of asymmetric
nitrene transfer processes including aziridination, sulfide imidation,
C–H amidation, and, most recently, C–H amination have
been demonstrated. The scopes of these biocatalytic carbene and nitrene
transfer reactions are often complementary to the state-of-the-art
processes based on small-molecule transition-metal catalysts, making
engineered biocatalysts a valuable addition to the synthetic chemist’s
toolbox. Moreover, enabled by the exquisite regio- and stereocontrol
imposed by the enzyme catalyst, this biocatalytic platform provides
an exciting opportunity to address challenging problems in modern
synthetic chemistry and selective catalysis, including ones that have
eluded synthetic chemists for decades.