An improved method for the separation of the genetic variants of a-L-fucosidase in human semen is described. The method involves, isoelectric focusing in ultrathin-layer PAG containing a mixture of ampholines, pH 5-7 and pH 3.5-9.5, and separator HEPES. The Fu pattern obtained is simple, easy to interpret, and reproducible. The occurrence of fucosidase phenotypes in 189 semen samples from the population of Wuhan was investigated, and the frequencies of the Fu alleles were calculated.Zusammenfassung. Die a-L-Fucosidase (Fn) der menschlichen Spermaproben wurde mittels einer verbesserten Isoelektrofokussierung in einer ultra-dt~nnen Schicht von Polyacrylamidgel (PAG) in einem gemischten pH-Bereich von 5-7 und 3.5-9.5 unter Zusatz von HEPES analysiert. Die erzielten Fu-Muster sind einfach und leicht interpretierbar und reproduzierbar. Bei 189 Spermaproben aus der Bev61kerung Wuhans wurde das Vorkommen der Fu-Ph~inotypen untersucht. Es wurden folgende Genfrequenzen berechnet: Fu 1 = 0.7857 und Fu 2 = 0.2143.
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