Embryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of domestic ungulates and large livestock species has been challenging. In contrast to ESCs that are usually established from the blastocyst, mouse expanded potential stem cells (EPSCs) are derived from four-cell and eight-cell embryos. We have recently used the EPSC approach and established stem cells from porcine and human preimplantation embryos. EPSCs are molecularly similar across species and have broader developmental potential to generate embryonic and extraembryonic cell lineages. We further explore the EPSC technology for mammalian species refractory to the standard ESC approaches and report here the successful establishment of bovine EPSCs (bEPSCs) from preimplantation embryos of both wild-type and somatic cell nuclear transfer. bEPSCs express high levels of pluripotency genes, propagate robustly in feeder-free culture, and are genetically stable in long-term culture. bEPSCs have enriched transcriptomic features of early preimplantation embryos and differentiate in vitro to cells of the three somatic germ layers and, in chimeras, contribute to both the embryonic (fetal) and extraembryonic cell lineages. Importantly, precise gene editing is efficiently achieved in bEPSCs, and genetically modified bEPSCs can be used as donors in somatic cell nuclear transfer. bEPSCs therefore hold the potential to substantially advance biotechnology and agriculture.

This paper proposes a line current distortion compensation method, which is a fusion of the conventional variable on-time (VOT) control and the average current mode (ACM) control, for DCM/CRM boost PFC converter with zero current switching (ZCS)/ zero voltage switching (ZVS)/ valley switching (VS)/ switching frequency limitation (SFL) functions. In this paper, the mathematical analyses of the line current distortion for the DCM/CRM boost PFC converter with ZCS/ZVS/VS/SFL functions are also derived to explain the causes of the line current distortion. In order to increase efficiency, the ZVS/VS/SFL functions are needed but the line current will be further distorted. To compensate the distorted line current, the ACM-based VOT (ABVOT) control for the DCM/CRM boost PFC converter is proposed. In addition, the constant-on-time-based noise-immunity S/H method is proposed to replace the multiplier, which is always needed for the conventional ACM-based control. The performance of the proposed ABVOT control with S/H method will be demonstrated by simulation and experiment results.Index terms -power factor correction (PFC), variable on-time (VOT), average current mode (ACM), multiplierless.

Healthcare professionals should reinforce patients' knowledge on appropriate responses to high and low blood glucose levels, intervene appropriately, discuss self-monitoring of blood glucose results with patients and track these results.

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