LiYbF4:Yb, Er nanoparticles have been successfully synthesized by thermal decomposition of multiple trifluoroacetic acid salts. The SEM and TEM results show the size of the LiYF4:Yb, Er nanoparticles is about 100 nm in diagonal line, and the morphology of the LiYF4:Yb, Er nanoparticles is highly uniform with octahedral structure. Under the excitation of 980 nm, the LiYF4:Yb, Er nanoparticles have higher upconversion luminescence efficiency compared with that of NaYF4:Yb, Er. The results indicate that the as-prepared LiYbF4:Yb, Er nanoparticles may have potential applications in bio-probes and displays.
In this paper, the biosynthesis of high-stable and biocompatible silver nanoparticles (AgNPs) was implemented by employing cell-free filtrate of Penicillium aculeatum Su1. The compositions analysis of reducing biomolecules in reaction system before and after AgNPs synthesis suggested
that proteins were mainly involved in the biosynthesis process of AgNPs. Polyacrylamide gel electrophoresis (SDS-PAGE) analysis displayed that two main protein bands with molecular weights ranging from 66.2 to 116 KDa and 35 to 45 KDa were capped on the surface of AgNPs. The further identification
of these protein bands by liquid chromatography-mass spectrometry (LC-MS/MS) analysis indicated that actin as a major protein component was responsible for stabilization of prepared AgNPs. The activity of nitrate reductase secreted by P. aculeatum Su1 was 73.73 ± 3.89 μg/(g
· h). Furthermore, the dialysis assay showed that small molecular components had significant impacts on yield and particle size of biosynthesized AgNPs. Reduced nicotinamide adenine dinucleotide or reduced nicotinamide adenine dinucleotide phosphate (NADH or NADPH)-dependent nitrate
reductases and other types of reductases or non-enzymatic bioactive molecules (≥ 3.5 KDa) might simultaneously participate in the biosynthesis process of AgNPs mediated by P. aculeatum Su1.
The structure of DNA molecules tethered to surfaces may significantly affect the efficiency of hybridization on the DNA microarray. Understanding the structure of single-stranded DNA (ssDNA) tethered to surfaces is critical for applying the molecular recognition function of DNA microarrays. Although a number of experimental methods have been applied to determine the structure of the DNA probe on surfaces, they can not provide enough information on the dynamical behavior of the ssDNAs on surfaces. Herein, we investigated the dynamics and interaction of seven DNA probes tethered on a silica surface by a molecular dynamics simulation. From the simulation results, we examined the structure and dynamics of the ssDNAs, by calculating the root-mean-square derivations, the tilt angles, the radius of gyration, and the distances of the neighboring ssDNAs. The data obtained from our simulation suggests the packing density has a significant effect on the overall structure and molecular orientation change of surface-tethered ssDNAs, which is complementary to the recent experimental reports. Our simulation provided a structural insight, which is helpful to better understand the behavior of ssDNA on surfaces and optimize the design of DNA microarray.
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