Photoacoustic (PA) imaging offers great promise for deep molecular imaging of optical reporters but has difficulties in imaging multiple molecular probes simultaneously in a strong blood background. Photoswitchable chromoproteins like BphP1 have recently allowed for sensitive PA detection by reducing high-blood background signals but lack multiplexing capabilities. We propose a method known as difference-spectra demixing for multiplexing multiple photoswitchable chromoproteins and introduce a second photoswitchable chromoprotein, sGPC2. sGPC2 has a far-red and orange state with peaks at 700 and 630 nm, respectively. It is roughly one-tenth the size of BphP1 and photoswitches four times as fast (2.4% per mJ∕cm 2). We simultaneously image Escherichia coli expressing sGPC2 and BphP1 injected in mice in vivo. Difference-spectra demixing obtained successful multiplexed images of photoswitchable molecular probes, resulting in a 21.6-fold increase in contrast-to-noise ratio in vivo over traditional PA imaging and an 8% to 40% reduction in erroneously demixed signals in comparison with traditional spectral demixing. PA imaging and characterization were conducted using a custom-built photoswitching PA imaging system.

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In this study, we applied small baseline subset-interferometric synthetic aperture radar (SBAS-InSAR) to monitor the ground surface deformation from 2017 to 2020 in the permafrost region within an ~400 km × 230 km area covering the northern and southern slopes of Mt. Geladandong, Tanggula Mountains on the Tibetan Plateau. During SBAS-InSAR processing, we inverted the network of interferograms into a deformation time series using a weighted least square estimator without a preset deformation model. The deformation curves of various permafrost states in the Tanggula Mountain region were revealed in detail for the first time. The study region undergoes significant subsidence. Over the subsiding terrain, the average subsidence rate was 9.1 mm/a; 68.1% of its area had a subsidence rate between 5 and 20 mm/a, while just 0.7% of its area had a subsidence rate larger than 30 mm/a. The average peak-to-peak seasonal deformation was 19.7 mm. There is a weak positive relationship (~0.3) between seasonal amplitude (water storage in the active layer) and long-term deformation velocity (ground ice melting). By examining the deformation time series of subsiding terrain with different subsidence levels, we also found that thaw subsidence was not restricted to the summer and autumn thawing times but could last until the following winter, and in this circumstance, the winter uplift was greatly weakened. Two import indices for indicating permafrost deformation properties, i.e., long-term deformation trend and seasonal deformation magnitude, were extracted by direct calculation and model approximations of deformation time series and compared with each other. The comparisons showed that the long-term velocity by different calculations was highly consistent, but the intra-annual deformation magnitudes by the model approximations were larger than those of the intra-annual highest-lowest elevation difference. The findings improve the understanding of deformation properties in the degrading permafrost environment.

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We analyze the mode evolution in mode-selective photonic lanterns with respect to taper lengths, affected by possible mode phase differences varying along the taper. As a result, we design a three-mode orbital angular momentum (OAM) mode-selective photonic lantern by optimizing the taper length with mode crosstalk below −24 dB, which employs only one single mode fiber port to selectively generate one OAM mode.