Age-related variations in genes and microRNAs expression and DNA methylation have been reported respectively; however, their interactions during aging are unclear. We therefore investigated alterations in the transcriptomes, miRNAomes and DNA methylomes in the same CD4+T cells from newborn (NB), middle-aged (MA) and long-lived (LL) individuals to elucidate the molecular changes and their interactions. A total 659 genes showed significantly expression changes across NB, MA and LL individuals, in which we identified four age-related co-expression modules with three hub networks of co-expressed genes and non-coding RNAs. Moreover, we identified 9835 differentially methylated regions (DMRs) including 7015 hypermethylated and 2820 hypomethylated DMRs in the NB compared with the MA, and 12,362 DMRs including 4809 hypermethylated and 7553 hypomethylated DMRs in the MA compared with the LL. The integrated analysis revealed a potential relationship between genes transcription and DNA methylation for many age- or immune-related genes, suggesting that DNA methylation-dependent transcription regulation is involved in development and functions of T cells during aging. Our results reveals age-related transcription and methylation changes and their interactions in human T cells from the cradle to the grave. Longitudinal work is required to establish the relationship between identified age-associated genes/DNA methylation and T cells aging phenotypes.
To analyze the feasibility of electrospinning nanofiber yarn using a wrapping yarn forming device, electrospun nanofiber-wrapped yarns and multiscale yarns were prepared by self-made equipment. The relationship between the surface morphology and properties of yarn and its preparation process was studied. The process parameters were adjusted, and it was found that some nanofibers formed Z-twisted yarns, while others showed exposed cores. To analyze the forming mechanism of electrospun nanofiber-wrapped yarn, the concept of winding displacement difference in the twisted yarn core A was introduced. The formation of nanofiber-wrapped structural yarns was discussed using three values of A. The starting point of each twist was the same position when A = 0 with a constant corner angle β. However, the oriented nanofiber broke or was pulled out from the gripping point when it was twisted, and it appeared disordered. The forming process of electrospun nanofiber-wrapped yarn displayed some unique phenomena, including the emission of directional nanofibers during collection, fiber non-continuity, and twist angle non-uniformity. The conclusions of this research have theoretical and practical value to guide the industrial preparation of nanofiber yarns and their wrapped yarns.
The current clinical audit project has made a significant improvement in establishing evidence-based practice of management of perineal pain among postpartum women in the gynecologic and obstetric hospital in Shanghai. It has been effective in increasing staff compliance and reducing the perineal pain among postpartum women.
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