In this paper, gold nanoparticles were combined with aflatoxin B1-aptamer as a single probe via the specific binding between aflatoxinB1 with aptamer, designing a colorimetric detection method. Meanwhile, an immunochromatographic strip with gold nanoparticles modified by complementary chains of aflatoxin B1 aptamer as the probe was developed for rapid detection of aflatoxin B1. Under the optimal conditions, the linear detection range of the colorimetric method was 0.5–50 ng/mL, the minimum detection limit was 0.5 ng/mL, the detection range of the immunochromatographic strip was 0.5–500 ng/mL, the minimum detection limit was 0.5 ng/ mL, and the semi-quantitative limit of detection (LOD) was 51 ng/mL. Finally, comparing the two methods, it is concluded that immunochromatographic strip detection is more sensitive, accurate and celerity. The recoveries of test samples were from 97.3–120%. All detections could be accomplished within 10 min, indicating that the strip could be a potentially effective tool for rapid on-site detections.
In this paper, gold nanoparticles were combined with aflatoxin B1-aptamer as a single probe via the specific binding between aflatoxinB1 with aptamer, designing a colorimetric detection method. Meanwhile, a chromatographic strip with gold nanoparticles modified by complementary chains of aflatoxin B1 aptamer as the probe was developed for rapid detection of aflatoxin B1. Under the optimal conditions, the linear detection range of the colorimetric method was 0.5–50 ng/mL, the minimum detection limit was 0.5 ng/mL, the detection range of the chromatographic strip was 0.5–500 ng/mL, the minimum detection limit was 0.5 ng/ mL, and the semi-quantitative limit of detection was 51 ng/ml. Finally, comparing the two methods, it is concluded that chromatographic strip detection is more sensitive, accurate and celerity. Sample recovery rate of the latter was from 97.3–120%. All detections could be accomplished within 10 min, indicating that the test strip could be a potentially effective tool for rapid on-site detections.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.