Dicalcium phosphate nanoparticles (nano-DCP) were synthesized by physical method using ball mill. The synthesized DCP nanoparticles were characterized by various techniques viz. Particle size analyser, Zeta potential, Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Energy Dispersive Analysis by X-ray (EDAX). The results revealed that synthesized nano-DCP had particle size between 10-20 nm and zeta potential value of -25.6±2.2 mV. Crystal size of nano DCP analysed by XRD was 29.31 nm. Calcium and phosphorus content of synthesized nano-DCP were 23.22 and 17.67 per cent. Ball milling, a popular physical method and is easy to operate, economic, time saving and higher yield. Based on its bioavailability, the synthesized feed grade nano DCP can be utilized for in ovo feeding of broiler embryos, livestock and poultry feeding.

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