Amylose content is a parameter that correlates with the cooking behavior of rice. It is measured at the earliest possible stages of rice improvement programs to enable breeders to build the foundations of appropriate grain quality during cultivar development. Amylose is usually quantified by absorbance of the amylose-iodine complex. The International Network for Quality Rice (INQR) conducted a survey to determine ways that amylose is measured, reproducibility between laboratories, and sources of variation. Each laboratory measured the amylose content of a set of 17 cultivars of rice. The study shows that five different versions of the iodine
Starches from six wild rice cultivars were studied for their chemical structures and physicochemical properties and compared with a long-grain rice starch. The six wild rice starches were similar in morphological appearance, X-ray diffraction patterns, swelling power, and water solubility index but different in amylose content, beta-amylolysis limit, branch chain length distribution, thermal properties, and pasting properties. The structure of the wild rice amylopectins was close to that of waxy rice amylopectin with more branching and a larger proportion of short branch chains of degree of polymerization 6-12 as compared with that of amylopectin from rice starch with a similar amylose content. The differences in branch chain length distribution of amylopectin and amylose content were assumed to contribute to the differences in physicochemical properties among the six wild rice starches as well as to the differences between the wild rice starches and the rice starch.
The effects of modification sequence on chemical structures and physicochemical properties of hydroxypropylated (HP) and crosslinked (XL) waxy maize starch were investigated. The physicochemical properties, including pasting, gelling, and thermal properties, were studied. The chemical structures of dual-modified starches and their beta-limit dextrins were characterized with high-performance liquid chromatography. The HP-XL starch had higher Brabender viscosity than did the XL-HP starch at both pH 7 and 3; however, both showed similar gelling properties. Significantly higher onset and peak gelatinization temperatures, gelatinization enthalpy, and lower retrogradation were observed for the HP-XL starch. The HP-XL starch also exhibited significantly higher beta-amylolysis limit and higher content of low molecular weight saccharides in its isoamylase-debranched starch, suggesting its structure was more accessible to enzymatic attack than the XL-HP starch. Structural analyses revealed different distribution patterns of modifying groups between the two modified starches. The results indicate that the modification sequence altered the susceptibility to enzymes, changed the locations of substitution, and modified the physicochemical properties of the HP and XL waxy maize starches.
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