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Ebola virus (EBOV) disease is a complex zoonosis that is highly virulent in humans and has caused many deaths. Highly sensitive detection of EBOV is of great importance for early-stage diagnosis for increasing the probability of survival. Herein, we established a cellular-beacon-mediated counting strategy for an ultrasensitive EBOV assay on a micromagnetic platform. The detection platform, which was assisted by on-demand magnetic-field manipulation, showed high integration and enhanced complex-sample pretreatment by magnetophoretic separation and continuous-flow washing. Cellular beacons (i.e., fluorescent cells) with superior optical properties were used as reporters, and each cellular beacon was used as a fluorescent tracking unit to quantify EBOV by counting the numbers of individual fluorescent signals on the micromagnetic platform. This method achieves high sensitivity with a detection limit as low as 2.6 pg/mL, and the detection limit shows little difference in a complex matrix. In addition, it has excellent specificity and good reproducibility. These results indicate that this method proposes an ultrasensitive detection strategy for early diagnosis of the disease.

To promote the development of nuclear power project in inland China, a specialized multi-criteria decision support framework is constructed for the site selection of inland nuclear power plants in this work. The best worst method (BWM), decision-making trial and evaluation laboratory (DEMATEL), and technique for order performance by similarity to ideal solution (TOPSIS) are integrated with Z-numbers, namely Z-BWM, Z-DEMATEL, and Z-TOPSIS. Z-BWM and Z-DEMATEL are combined to produce the weights of criteria, and an extended Z-TOPSIS is utilized to determine the ranking of all alternatives. Finally, a case study is performed in Hunan province to demonstrate the practicability of the proposed decision support framework. The robustness and feasibility of the proposed method are shown by an in-depth analysis of the decision results.that are not prone to tsunamis or typhoons. China has a total of 48 nuclear power units in operation, but they are all in coastal areas. The specific research on the site selection of China's inland NPPs is still insufficient. In this work, a multi-criteria decision support framework is provided to identify the best site for inland NPPs in China, and the contributions are summarized as follows:(1) The existing researches on multi-criteria decision-making (MCDM) problems inevitably involve the uncertainly and unreliability of human preferences. Therefore, Z-number is introduced into the proposed MCDM framework in this work, and all evaluation information is given using Z-number linguistic variables.(2) Best worst method (BWM) and decision-making trial and evaluation laboratory (DEMATEL) are extended to Z-numbers, i.e., Z-BWM and Z-DEMATEL. The hybrid model not only considers the impact of the criterion relations on the criterion weights, but also provides a long-term improvement for inland NPP site selection.(3) The technique for order performance by similarity to ideal solution (TOPSIS) is combined with Z-numbers, namely Z-TOPSIS. It can effectively reduce the influence of outliers, and provide more reliable ranking results than the classic TOPSIS.(4) Currently, few researches focus on the site selection of China's inland NPPs. Based on the reactor siting criteria developed by IAEA, a specialized evaluation system considering the eco-environment, geographic features, and socio-economic factors in China's interior is constructed.The structure of the paper is organized as follows. Section 2 reviews some studies related to NPP site selection. Section 3 introduces some basic concepts of Z-numbers. Section 4 presents the proposed framework for inland NPP site selection. Section 5 provides a case study in Hunan province of China. Section 6 conducts an in-depth analysis concerning selection results. Finally, Section 7 draws conclusions.

BackgroundAdequate operation interspace is the premise of laparoscopy, and carbon dioxide (CO2) was an ideal gas for forming lacuna. A retroperitoneal space is used to form operation interspace in retroperitoneal laparoscopic radical nephrectomy by making ballooning, and the retroperitoneal space has no relative complete and airtight serous membrane, therefore CO2 absorption may be greater in retroperitoneal than transperitoneal laparoscopic radical nephrectomy. Excess CO2 absorption may induce hypercapnemia and further cause physiopathological change of respiratory and circulatory system. Therefore, exact evaluation of amount of CO2 which is eliminated from body via minute ventilation is important during retroperitoneal laparoscopic radical nephrectomy. The aim of the paper is to study the correlation between CO2 storage at the last minute of gas insufflation and area of retroperitoneal lacuna during retroperitoneal laparoscopic radical nephrectomy.MethodsForty ASA I/II patients undergoing retroperitoneal laparoscopic radical nephrectomy were enrolled. CO2 storage at the last minute of gas insufflation and area of a retroperitoneal lacuna were observed. Linear correlation and regression were performed to determine the correlation between them.ResultsThere was positive correlation between CO2 storage at the last minute of gas insufflation and area of retroperitoneal lacuna (r = 0.880, P = 0.000), and the equation of linear regression was y = −83.097 + 0.925x (R2 = 0.780, t = 11.610, P = 0.000).ConclusionsAmount of CO2 which is eliminated from body via mechanical ventilation could be calculated by measuring the area of retroperitoneal lacuna during retroperitoneal laparoscopic radical nephrectomy, and an anesthetist should be aware of the size of lacuna to predict high CO2 storage at the last minute of gas insufflation.

No abstract

Under the double pressure of global energy consumption and climate change, nuclear power has become a low-carbon alternative energy source that could transform the energy structure of the globe. In the nuclear power industry, selecting suitable suppliers plays a significant role in improving the overall performance of nuclear power projects. Along with this symmetrical impact, this paper aims to develop a multistage decision-support framework to determine the optimal nuclear power equipment supplier, which is constructed in the context of Z-number information. Concretely, the Analytic Network Process (ANP) and Tomada de Decisão Iterativa Multicritério (TODIM) are extended by Z-numbers symmetrically—namely, Z-ANP and Z-TODIM. Z-ANP is first applied to analyze the symmetrical interdependence of criteria, so as to accurately determine the criterion weights. Further, the ranking of alternatives is obtained by Z-TODIM, which sufficiently considers the risk preference and psychological states of decision-makers. Finally, a practical case of nuclear-grade cable procurement in the Karachi 2-3 international nuclear power project is performed to illustrate the practicality of the proposed method, and its robustness and superiority are proven by comparing it with current representative approaches.

Three isopimarane diterpenes [fladins B (1), C (2), and D (3)] were isolated from the twigs and leaves of Chinese folk medicine, Isodon flavidus. The chemical structures were determined by the analysis of the comprehensive spectroscopic data, and the absolute configuration was confirmed by X-ray crystallographic analysis. The structures of 1–3 were formed from isopimaranes through the rearrangement of ring A by the bond break at C-3 and C-4 to form a new δ-lactone ring system between C-3 and C-9. This structure type represents the first discovery of a natural isopimarane diterpene with an unusual lactone moiety at C-9 and C-10. In the crystal of 1, molecules are linked to each other by intermolecular O-H···O bonds, forming chains along the b axis. Compounds 1–3 were evaluated for their bioactivities against different diseases. None of these compounds displayed cytotoxic activities against HCT116 and A549 cancer cell lines, antifungal activities against Trichophyton rubrum and T. mentagrophytes, or antiviral activities against HIV entry at 20 µg/mL (62.9–66.7) µM. Compounds 1 and 3 did not show antiviral activities against Ebola entry at 20 µg/mL either; only 2 was found to show an 81% inhibitory effect against Ebola entry activity at 20 µg/mL (66.7 µM). The bioactivity evidence suggested that this type of compound could be a valuable antiviral lead for further structure modification to improve the antiviral potential.

C20H18O8, monoclinic, P21/c (no. 14), a = 7.3855(2) Å, b = 28.1424(9) Å, c = 8.5863(3) Å, β = 98.4040(10)°, V = 1765.46(10) Å3, Z = 4, R gt (F) = 0.0545, wR ref (F 2) = 0.1641, T = 273(2) K.