We found that the 5' nontranslated leader sequence from encephalomyocarditis virus (EMCV) allowed transcripts that were synthesized by the T3 RNA polymerase in mammalian cells to be translated in a cap-independent fashion. Stable mouse cell lines that carry the T3 RNA polymerase gene expressed the chloramphenicol acetyltransferase (CAT) It has been shown that the bacteriophage T3 and T7 RNA polymerases can function in mammalian cells and can transcribe DNA that is located in either the nucleus or the cytoplasm
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