The effect that egg yolk or maternal n-3 FA have on the cardiac tissue long-chain n-3 FA status of chicks during growth was investigated. Eggs with low, medium, and high levels of n-3 PUFA were obtained by feeding breeder hens a wheat/soybean meal-based diet containing 5% sunflower oil (Low n-3), 2.5% sunflower oil plus 2.5% fish oil (Medium n-3), or 5% fish oil (High n-3). The chicks hatched from Low, Medium, and High n-3 eggs were fed a diet containing 18:3n-3, but devoid of long-chain n-3 FA. The FA composition of cardiac tissue was determined on days 0, 14, 28, and 42. At day 0, the cardiac FA reflected maternal diet. With time, the level of all the long-chain n-3 FA decreased compared with day 0, and this was true especially by day 14. These data show that dietary 18:3n-3 fed to the chicks did not sustain high levels of EPA and DHA in cardiac tissue, despite the high content of long-chain n-3 FA in the maternal diet. At days 0 and 14, the chicks hatched from High and Medium n-3 eggs had higher 20:5n-3, 22:5n-3, and 22:6n-3 contents with a concomitant reduction in 20:4n-6 in the cardiac tissue compared with the Low n-3 egg group. Cardiac tissue of birds hatched from Medium n-3 eggs retained higher levels of 20:5n-3 up to day 42 of growth when compared with other treatments (P < 0.05). None of the treatments was effective in maintaining DHA levels after day 14 of growth.
Eggs with diverse ω–6/ω–3 ratio produced by feeding breeder hens a wheat-soybean meal-basal diet containing 5% (wt/wt) sunflower oil (Hω6), 5% fish oil (Hω3) or 2.5% sunflower oil plus 2.5% fish oil (Mω3ω6) were incubated. The hatched chicks were fed a docosahexaenoic acid (DHA)-deficient diet up to 6 weeks of age. The fatty acid composition of chick brain was determined on 0, 2, 4 and 6 weeks and brain weight was taken on day 0 and day 42. The ω–6/ω–3 ratios were 37.12, 4.21 and 0.98 for the maternal diet; 28.36, 2.83 and 0.89 for the egg yolk; 1.94, 0.48 and 0.18 for hatched chick brain (p < 0.05). At 2 weeks of age, the ω–6/ω–3 ratios were 1.88, 0.81 and 0.60 for chicks hatched from hens fed Hω6, Mω3ω6 and Hω3 diets, respectively. The brain DHA contents at 0 and 2 weeks of age were Hω3 > Mω3ω6 < Hω6 (p < 0.05) and at 4 and 6 weeks of age Hω3 = Mω3ω6 > Hω6. Dietary C18:3ω3 in the starter and finisher diet did not increase brain DHA (p > 0.05). The significant increase in the content of C22:5ω3 at 6 weeks of age in group 1 birds with a concomitant reduction in DHA suggests a weak delta-4 desaturation but an effective delta-6 and delta-5 desaturation similar to human infants. Considering the role of DHA in early brain development and growth, the maternal supply of DHA during growth might be of importance when fed a DHA-deficient neonatal diet.
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