. 2008. Effects of ruminal fermentation, intestinal digestion and fecal contact on the survival of Fusarium graminearum on colonized barley grain. Can. J. Plant Sci. 88: 143Á146. Barley grains were colonized in the laboratory with a mixture of three Fusarium graminearum (FG) strains and processed in five different ways. The survivability of FG on the surface of barley kernels in the rumen, lower digestive tract and after contact with feces was investigated. FG-colonized kernels were placed in nylon bags and incubated in the rumen of three steers for 0, 6, 12 and 24 h. Viability of FG was reduced (P B0.001) after 6 h and FG was non-viable after 12 h in the rumen. To investigate viability in the lower digestive tract, FG-colonized kernels were placed in nylon bags, passed through the duodenum of three cows and recovered in the feces. No viable FG was found in any of the kernels that passed through the lower digestive tract. FG-colonized kernels were also embedded in cattle feces for 0, 2, 4, 7, 14 and 21 d. FG viability was reduced (PB0.001) after 2 d and was not detected after 4 d of contact with feces. The likelihood of FG remaining viable after passage through the intestinal tract and excretion in the feces of cattle is exceedingly low. Ensuite ils ont e´tudie´la survie de FG a`la surface des grains dans le rumen, la partie infe´rieure du syste`me digestif et apre`s contact avec les fe`ces. Les grains colonise´s par FG ont e´te´place´s dans des sacs en nylon puis incube´s 0, 6, 12 et 24 h dans le rumen de trois bouvillons. La viabilite´de FG diminue (PB0,001) apre`s 6 h d'incubation et disparaıˆt 12 h plus tard dans le rumen. Pour ve´rifier la viabilite´dans la partie infe´rieure du syste`me digestif, les auteurs ont place´les grains colonise´s par FG dans des sacs en nylon qu'ils ont fait passer dans le duode´num de trois vaches avant de les re´cupe´rer dans les fe`ces. Aucun des grains traite´s de cette manie`re ne portait de FG viable. Les grains colonise´s par FG ont aussi e´te´plonge´s dans des fe`ces de bovins pendant 0, 2, 4, 7, 14 et 21 j. La viabilite´de FG diminue (PB0,001) au bout de deux jours et disparaıˆt apre`s 4 j de contact avec les matie`res fe´cales. La probabilite´que FG demeure viable apre`s avoir traverse´le tube digestif des bovins et eˆtre excre´te´dans les fe`ces est extreˆmement faible.
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