Soybean and cottonseed meals were subjected to various treatments with enzymes, heat, and chemicals, and their effects on hydrolysis and removal of phytic acid were studied. Soybean meal and cottonseed meal contain about 2.2 and 4.4% (w/w) phytate, of which 60 and 50%, respectively, were in water-soluble forms. The water-insoluble portion of the phytate was further hydrolyzed and removed by extraction with acid, application of enzymes, precipitation by divalent cations, and autolysis by endogenous phytase. Washing with 1 N HC1 removed about 87% of phytate in soybean meal, while treatment with cationic agents had little effect on removing phytate from the plant seed. Coapplication of phytase with cellulose
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