The mechanisms responsible for the rapid increases in levels of gonadotropin and steroid hormones upon administration of cisplatin to rats are not clearly understood. In the present study, the morphologic effects of cisplatin on the testis and the relationship of these changes to serum hormone levels were studied in adult rats. Upon administration of a single dose (5 mg/kg) of cisplatin, the levels of testosterone in the peripheral blood decreased and the level of luteinizing hormone (LH) increased. After 3 days, significant damage to the germinal epithelium became detectable by morphologic examination. In Ley-dig and Sertoli cells, dilatation of the endoplasmic reticulum, structural changes in mitochondria and an increase in the numbers of inclusion bodies were detected. Although blood-testis barriers between Sertoli cells were normal, the relationship between germinal cells and the relationship between germinal cells and Sertoli cells were distorted. It is concluded that damage to Ley-dig cells and withdrawal of the controlling effects of the germinal epithelium on the LH-Leydig cell axis could explain the changes in serum hormone levels.

Microscopic ovary features of Zebrafish were analyzed. Samples were fixed in Bouin's solution, mounted in parafin and cut into 6-7 µm-thick slices which were stained with Hematoxylin and Eosin (HE), methylene blue and using Periodic-acid Schiff method (PAS). The thin sections in ultramicrotome with the thickness of approximately 0,5-0,7 µm were examined by using Jeol electron microscope following contrasting with Uranyl Acetate-Lead Citrate. The zebrafish was an extremely dynamic organ in which follicles underwent asynchronous development. The oocytes of Zebrafish ovary were observed in various phases. The oocyte development of zebrafish was divided into four stages (primary growth, cortical alveolus, vitellogenic and mature oocyte). Oocyte diameters were observed to vary between 0.,08 mm and 0.76 mm.

Cavernous tissues of 27 impotent and 4 potent men were evaluated in order to determine the ultrastructural changes in patients with vasculogenic impotence. Tissue samples were obtained during penile prosthesis insertions and Surgical repairs of penile fractures in impotent and potent patients, respectively. For the clinical evaluation (pharmacologic color Doppler ultrasound, dynamic pharmacocavernosometry and nocturnal penile tumescence monitoring) patients were divided into three groups: slight/moderate arterial dysfunction (n = 5), severe arterial dysfunction (n = 9) and purely cavernous erectile dysfunction (n = 9). No significant ultrastructural alteration was noticed in the group with moderate arterial dysfunction; decrease and deterioration of smooth muscle cells was the prominent finding in the severe arterial and venogenic dysfunction groups. Preliminary examination of cavernous tissue may be a part of routine evaluations in the approach to the management of erectile dysfunction.

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