The objective of our experiment was to study the histrostructural changes in the ovaries of pregnant rabbits with previous reproductive cycles in artificial insemination without using further hormonal treatment to increase the reproductive function of rabbits. We have studied the ovaries of pregnant rabbits of the Hyla breed on the 7th day of pregnancy age, which were in the fifth reproductive cycle. The AI technique in rabbits needs an ovulation stimulator or inducer because female rabbits are induced ovulators. In our experiments, we used Surfagon for induction of ovulation by the method proposed by Lisin V. I., Sushko A.B., Institute of animal's science of the NAAS. The ovaries were fixed in 10%-neutral formalin solution. Paraffin histosections were made and coloured with hematoxylin and eosin according to the standard technique.The macroscopic and microscopic studies of the rabbit ovary of the 5th reproductive cycles indicate a high level of their luteinization. The results of the histological study show that the histostructural picture of the ovaries of pregnant rabbits reflects the nature of the course of the phases of the previous reproductive cycles typical for this animal species. In the ovary of pregnant rabbits of the fifth reproductive cycle, all generations of follicles were found. The predominant functional elements of the ovarian parenchyma were luteal structures: the corpus luteum of pregnancy, atretic bodies and interstitial glandular tissue. There were two types of follicular atresia: obliterating, which took place in the preantral (primary and secondary) follicles and cystic and the antral tertiary follicles. The formation of hemorrhagic follicles was a type of cystic atresia. The formation of atretic bodies occurred as a result of luteinization of primary and secondary follicles. In the ovaries of pregnant rabbits of the fifth reproductive cycle, yellow bodies of various generations were found, that is the evidence of their functioning for several reproductive cycles. The number and development of corpus luteum in the right and left ovaries was asynchronous, which indicates the asynchronous nature of ovulation in the preceding and current reproductive cycles. Interstitial glandular tissue was the predominant histological element in the ovaries of rabbits of the fifth reproductive cycle. Compared with the ovaries of other mammalian species, it was found not only in the cortical, but also in the medulla of the ovaries of pregnant rabbits.
In commercial rabbit meat production, the combination of artificial insemination and management in a batch system is widely used and has replaced natural mating. However, in such systems the success of AI depends largely on the synchronization of oestrus. The irregularity of the oestrus cycle, especially during the lactation period, necessitates a method to synchronize sexual receptivity of the does on the intended AI day. The objective of our experiment was to compare the efficacy of ovulation stimulation in artificial insemination of female rabbit. The task was to compare the results of artificial insemination by using Pregnant Mare Serum Gonadotropin "Follimag" (Mosagrogen) and non-hormonal treatment of (PMSG). The AI technique in rabbits needs an ovulation stimulator or inducer because female rabbits are induced ovulators. In our experiments, we used Surfagon to inducte ovulation by the methodology proposed by Lisin V. I., Sushko A.B., Institute of animal science of the NAAS. Experimental female rabbits were divided into 3 homogenous groups. All animals were maintained and fed in the same way. Artificial light was on from 09.00 to 20.00 h. Receptivity of all does was judged based on vulva colour and turgency just before gonadotropin treatment and in the day before artificial insemination. In two experimental groups with PMSG treatment was observed more pronounced improvement of vulva colour and turgency the day before artificial insemination. Gonadotropin treatment was applied intramuscularly once three days before artificial insemination. We used the dose of 40IU of PMSGfirst group, 25IU of PMSG-second group and control group (no oestrus synchronization). The experimental data have shown that the differences between the results of artificial inseminations of rabbit does with the serial use of "Follimag" (PMSG) resulted in 19,29% and 11,36% improvement as compared to the controls (40 IU of PMSG-75,0%;25 12 IU of PMSG-82,93%;control group-63,64%). In the future carry out further research to monitor the effects of gonadotropin hormones on fertility, estrus, quality and quantity of litter, the physiological condition of rabbit does.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.