BACKGROUND:There is a fairly high demand of patients for procedures to improve the appearance and rejuvenation of the skin, while the methods used have a different, often aggressive principle of action, requiring a long period of rehabilitation. AIM:The purpose of the work was to determine the safety and effectiveness of exposure to the skin in order to improve its quality and rejuvenate such methods as biorevitalization and fractional photothermolysis using an erbium laser, to determine changes in the structural components of the skin that affect its condition, before and after exposure to various types of procedures. MATERIALS AND METHODS:40 patients were examined, who were divided into two groups. Patients of the first group received the biorevitalization procedure, the second group of patients underwent fractional photothermolysis. A fragment of skin was taken from all patients before and after the procedures and the volumetric density of collagen types I and III, laminin, growth factors (VEGF, FGF, TGF, IGF) in the dermis and epidermis, interleukins (IL-4, IL-1) in the dermis and epidermis was assessed. We assessed the level of changes in these indicators before and after procedures in different groups, correlation analysis. RESULTS:An increase in the volumetric density of collagens, laminin, growth factors, interleukins was recorded, but the correlations in different groups were different. The structural parameters of the skin during biorevitalization positively correlated with growth factors, and with fractional photothermolysis with indicators of local immunity, such as interleukins, TGF. CONCLUSION:It was concluded that both biorevitalization and laser exposure have a positive effect on the quality of the skin, but in the case of revitalization, these changes occur through the stimulating action of hyaluronic acid, the entrainment of growth factors in the skin, and in the case of fractional photothermolysis, the formation of new collagen, strengthening of the dermo-epidermal connections occur through the processes of inflammation and regeneration.
The study is devoted to the study of the content of collagen types I and III, laminin in the epidermis and dermis, insulin-like and vascular growth factors in the skin of women with a preserved menstrual cycle and women in menopause. A study was also made of the content of the same indicators after cosmetic procedures aimed at improving the quality of the skin. The degree of effectiveness of cosmetic procedures for skin regeneration was determined depending on the presence or absence of menopause.
Introduction. Ablative fractional laser photothermolysis (A-FLPh), used for rejuvenation of aging skin, is based on its controlled damage. Factors of the immune system are involved in the reparative regeneration processes triggered, which, in turn, is also subject to age-related remodeling or immunostaining. The aim of the work was to evaluate in dynamics the response of the immune system to the A-FLPh procedure performed for correction of age-associated facial skin changes. Materials and methods. The study included 25 women aged 42 to 55 years who underwent A-FLPh treatment of facial skin with an Erbium laser. The number of leukocytes, monocytes, neutrophils, lymphocytes, T-lymphocytes, T-helpers, cytotoxic T-cells, regulatory T-cells, NKT-lymphocytes, NK-lymphocytes were counted in the peripheral blood before, on the 8th and 24th after the procedure. We studied phagocytic function of neutrophils and monocytes, NBT-reducing and lysosomal activity of neutrophils; determined the amount of IgA, IgM, IgG, IL-4, IL-6, IL-8, IL-10, circulating immune complexes (CIC). Results. On the 8th day after A-FLPh, the number of neutrophils, neutrophils and monocytes phagocytosis, the number of lymphocytes, regulatory T-cells, IL-6 and IL-8 levels significantly increased; in parallel, the number of CD11b+ NK-lymphocytes, CD11b+ NKT-lymphocytes, IgA, IgG, IL-10 concentrations decreased. On the 24th day, quantitative functional indices of neutrophils, total number of lymphocytes, concentrations of IgA and IgG had no reliable difference from pre-procedure values, phagocytic parameters of monocytes, number of regulatory T-cells, IL-6 and IL-8 levels remained significantly higher, while the number of CD11b+ NK-lymphocytes, CD11b+ NKT-lymphocytes and IL-10, on the contrary, significantly lower than the initial level. Discussion. The revealed changes of systemic immunity indices after A-FLPh testify to both direct and regulatory-modulatory influence of immune factors on skin repair and remodeling after laser damage. Conclusion. The A-FLPh procedure induces a response from both cellular and humoral factors of the immune system, predominantly innate immunity.
The use of lasers in medical cosmetology today is widespread. The effects of laser techniques are pronounced and persistent. It is known that skin microcirculation increases after the procedures, renewal of keratinocytes and fbroblasts is stimulated, but there is little information about the molecular basis of the action of ablative lasers, about the mechanisms underlying the formation of new tissues (various types of collagens, laminin, vessels of the microvasculature).Purpose of the study. Formation of a detailed understanding of the mechanisms underlying the regenerative processes of the skin after ablative laser exposure.Results. After ablative laser exposure, the amount of interleukin-1α signifcantly increases, which leads to stimulation of the formation of type I and III collagens, laminin. There is an increase in vascular, fbroblast growth factor, which also helps to stimulate the proliferation of fbroblasts, increase its blood supply.Conclusions. Based on the data obtained, it can be concluded that fractional photothermolysis improves the elastic properties of the skin, enhances skin trophism and activates the metabolism of the skin. The prolongation of the effects of a single procedure is due to the activation and stimulation of the skin’s own regenerative resources, which contributes to an increase in its own fbroblasts, collagens, vessels of the microvasculature, cells of the epidermis and dermis.
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