The first two authors contributed equally to this work Although several studies have shown physiological functions of interleukin (IL)-32, the role of IL-32 in liver has not yet been reported. This study was initiated to examine the effects of IL-32y on APAPinduced acute hepatic failure in IL-32y transgenic mice. IL-32y overexpressing and non-transgenic mice received 500 mg/kg Acetoaminophen (APAP) intraperitoneally. Serum alanine transaminase and aspartate transaminase were significantly lower in the APAP treated IL-32y overexpressing mice compared with those APAP-treated non-transgenic. IL-32y markedly reduced a restricted area of the necrosis and inflammation. APAP-induced reduced glutathione depletion, induction of nitric oxide and lipid peroxidation, and cytochrome P4502El expression was significantly lowered in the IL-32y overexpressing mice. Elevation of Kupffer cells and natural killer cells by APAP were reduced in the IL-32y overexpressing mice. The expression ofIL-la, IL-lra, macrophage inflammatory protein-2, C-C motif chemokine ligand 5 and tissue inhibitor of metalloproteinase-l was increased by APAP in nontransgenic mice, and were lowered in the IL-32y overexpressing mice. Moreover, APAP-induced nuclear transcription factor-kappa B (NF-KB) and signal transducers and activators oftranscription 1 (STATl) activities were greatly lowered in the IL-32y overexpressing mice. The results indicate that IL-32y could effectively inhibit drug-induced hepatic failure, and reduce the number of cytotoxic immune cells and pro-inflammatory cytokine production through reduced activities of NF-KB and STATl. This might be attributable to lowering APAP-induced liver toxicity in IL-32y overexpressing mice.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.