Cytochrome P450 (CYP) epoxygenases convert arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EET), which exert diverse biological activities in a variety of systems. We previously reported that the CYP2J2 epoxygenase is overexpressed in human cancer tissues and cancer cell lines and that EETs enhance tumor growth, increase carcinoma cell proliferation, and prevent apoptosis of cancer cells. Herein, we report that CYP epoxygenase overexpression or EET treatment promotes tumor metastasis independent of effects on tumor growth. In four different human cancer cell lines in vitro, overexpression of CYP2J2 or CYP102 F87V with an associated increase in EET production or addition of synthetic EETs significantly induced Transwell migration (4.5-to 5.5-fold), invasion of cells (3-to 3.5-fold), cell adhesion to fibronectin, and colony formation in soft agar. In contrast, the epoxygenase inhibitor 17-ODYA or infection with the antisense recombinant adeno-associated viral vector (rAAV)-CYP2J2 vector inhibited cell migration, invasion, and adhesion with an associated reduction in EET production. CYP overexpression also enhanced metastatic potential in vivo in that rAAV-CYP2J2-infected MDA-MB-231 human breast carcinoma cells showed 60% more lung metastases in athymic BALB/c mice and enhanced angiogenesis in and around primary tumors compared with control cells. Lung metastasis was abolished by infection with the antisense rAAV-CYP2J2 vector. CYP epoxygenase overexpression or EET treatment up-regulated the prometastatic matrix metalloproteinases and CD44 and down-regulated the antimetastatic genes CD82 and nm-23. Together, these data suggest that CYP epoxygenase inhibition may represent a novel approach to prevent metastasis of human cancers. [Cancer Res 2007;67(14):6665-74]

This paper reviews recent developments in the field of current measurement which employ a wide range of optical and fiber optic techniques. Depending on the current sensing mechanisms involved and the sensing materials used, optical current sensors (OCSs) may be categorized into four main groups: (i) OCSs employing optical fiber as their sensing elements, (ii) OCSs using bulk glass to sense the current, (iii) OCSs using electro-optic hybrid sensing devices, and (iv) OCSs using magnetic field sensing devices. The operational modes of a variety of OCSs have been grouped and discussed, and several examples given. It can be seen that as a result of an intensive and wide ranging research effort using various approaches, substantial progress in the differing aspects of the optical current sensing techniques considered, such as the sensing material used, the configurations of sensing elements introduced, and the detection schemes adapted, has been achieved during the past years. An overall view of the field shows it to be as an active and exciting research area, highlighting several recently introduced and novel sensing materials and configurations which provide impressive results in this field of instrumentation. Examples of applications for the electric power industry are discussed more extensively.

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We report a novel technique in which by combining the output from two multimode laser diodes a synthesized source with an extremely short coherence length is produced for use in white-light interferometric sensing systems. Experimentally and theoretically it is demonstrated that, by summing the autocorrelation function of two multimode laser diodes with a wavelength difference of 108 nm, a synthesized source with an equivalent coherence length of ~4 microm is generated, greatly reducing the signal-to-noise ratio required to identify the central fringe position.

ObjectiveTo enhance theoretical support of pyogenic liver abscess (PLA) treatment by comparing characteristics of patients with either PLA with an identified infectious origin (non-cryptogenic) or PLA with no obvious underlying cause (cryptogenic).MethodsThis retrospective study included all first episodes of PLA in adults admitted to a tertiary hospital between 2009 and 2016. Relevant clinical data were collected for patients with cryptogenic or non-cryptogenic PLA and compared across a number of characteristics.ResultsIn all, 178 patients were included: 111 cases (62.4%) of cryptogenic PLA, and 67 cases (37.6%) of non-cryptogenic PLA. Diabetes mellitus was significantly more prevalent in patients with cryptogenic PLA than those with non-cryptogenic PLA. The proportion of multidrug resistance/poly-microbial infection was significantly lower and Klebsiella pneumoniae infection was significantly higher in the cryptogenic versus non-cryptogenic PLA group. Metastatic infection occurred in four patients with cryptogenic PLA only, and all had diabetes and K. pneumoniae infection. Multivariate logistic regression analysis revealed that male sex, diabetes and K. pneumoniae were independent predictors for cryptogenic PLA.ConclusionsCryptogenic and non-cryptogenic PLA have distinctly different characteristics, suggesting a potential need for different treatment approaches.