Abstract. In the Asian monsoon region, variations in the stable isotopic composition of speleothems have often been attributed to the "amount effect". However, an increasing number of studies suggest that the "amount effect" in local precipitation is insignificant or even non-existent. To explore this issue further, we examined the variability of daily stable isotopic composition (δ 18 O) in precipitation from September 2011 to November 2014 in Nanjing, eastern China. We found that intra-seasonal variations of δ 18 O during summer were not significantly correlated with local rainfall amount but could be linked to changes in the moisture source location and rainout processes in the source regions. Our findings suggest that the stable isotopes in summer precipitation could signal the location shift of precipitation source regions in the inter-tropical convergence zone (ITCZ) over the course of the monsoon season. As a result, changes in moisture source location and upstream rainout effect should be taken into account when interpreting the stable isotopic composition of speleothems in the Asian monsoon region. In addition, the temperature effect on isotopic variations in non-monsoonal precipitation should also be considered because precipitation in the non-monsoon season accounts for about half of its annual precipitation.

Hong Kong is one of the most high‐rise and highly compact cities in the world. The urban land surface is highly heterogeneous, which creates low‐level convergence zones in urban areas, particularly the Kowloon Peninsula. The low‐level convergence zone is due to the combined effect of urban heat island circulation (UHIC) and sea‐land breeze circulation (SLBC) under weak northeasterly synoptic flow. To study the impacts of anthropogenic fluxes and built‐up areas on the local circulation, the Weather Research and Forecasting (WRF) mesoscale model is combined with the multilayer urban canopy building effect parameterization/building energy model (BEP/BEM) parameterization to produce a 3 day simulation of an air pollution episode in Hong Kong in September 2012. To better represent the city land surface features, building information is assimilated in the central part of the Kowloon Peninsula. The WRF‐BEP‐BEM model captures the 2 m temperature distribution and local wind rotation reasonably well but overestimates the 10 m wind speed with a mean bias error of 0.70 m/s. A dome‐shaped feature with a high level of moisture is captured in the convergence zones due to intensified UHIC and inflowing SLBC. The anthropogenic heat increases the air temperature by around 0.3°C up to 250 m, which in turn modifies the SLBC. A new drag coefficient based on λP, plan area per unit ground area, is tested. Besides the basic physical characteristics captured by the WRF‐BEP‐BEM model, the stagnation of wind in the lower level convergence zone is better captured by this approach than by the traditional constant value coefficient.

The human pathogenic bacterium Helicobacter pylori has been ascertained to be an aetiological agent for chronic active gastritis and a significant determinant in peptic and duodenal ulcer diseases. Endophytic metabolites are being recognized as a versatile arsenal of antimicrobial agents, since some endophytes have been shown to possess superior biosynthetic capabilities owing to their presumable gene recombination with the host, while residing and reproducing inside the healthy plant tissues. A total of 32 endophytic fungi isolated from the medicinal herb Cynodon dactylon (Poaceae) were grown in in vitro culture, and the ethyl acetate extracts of the cultures were examined in vitro for the anti-H. pylori activity. As a result, a total of 16 endophyte culture extracts were identified as having potent anti-H. pylori activities. Subsequently, a detailed bioassay-guided fractionation of the extract of the most active endophyte (strain number: CY725) identified as Aspergillus sp., was performed to afford eventually four anti-H. pylori secondary metabolites. The four isolated compounds were identified through a combination of spectral and chemical methods (IR, MS, 1 H-and 13 C-NMR) to be helvolic acid, monomethylsulochrin, ergosterol and 3b-hydroxy-5a,8a-epidioxy-ergosta-6,22-diene with corresponding MICs of 8.0, 10.0, 20.0 and 30.0 lg/ml, respectively. The MIC of ampicillin co-assayed as a reference drug against H. pylori was 2.0 lg/ml. Furthermore, preliminary examination of the antimicrobial spectrum of helvolic acid, the most active anti-H. pylori metabolite characterized from the endophyte culture, showed that it was inhibitory to the growth of Sarcina lutea, Staphylococcus aureus and Candida albicans with MICs of 15.0, 20.0 and 30.0 lg/ml, respectively.

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Aims:  This study was conducted to measure the efficacy of the marine antagonist Rhodosporidium paludigenum in the suppression of postharvest decay of Chinese winter jujube caused by Alternaria alternata and to explore the possible mode of action involved. Methods and Results:  The efficacy of controlling postharvest diseases by R. paludigenum was examined. Rapid yeast colonization of wounds was observed during the first 48 h at 25°C. The yeast at 1 × 108 cells ml−1 of washed cells suspension provided better control of A. alternata than any other treatment. The concentration of the antagonist had significant effects on biocontrol effectiveness: as the concentration of R. paludigenum was increased, the disease incidence decreased. Meanwhile, R. paludigenum significantly inhibited the natural development of decay and did not damage fruit quality parameters including lightness values, hue angle, firmness, soluble solids, ascorbic acid and titratable acidity in 21 days’ storage at 25°C. Conclusions:  Rhodosporidium paludigenum was effective in controlling postharvest decay of Chinese winter jujube and did not impair fruit quality parameters. Significance and Impact of the Study:  Rhodosporidium paludigenum can be used as a nonchemical agent in postharvest biological control of Chinese winter jujube.