. 2009. Effect of dietary crude protein level on visceral organ mass, cellularity, and the protein expression of ATP synthase, Na /K -ATPase, proliferating cell nuclear antigen and ubiquitin in feedlot steers. Can. J. Anim. Sci. 89: 493Á501. Twenty-four steers [initial body weight (BW) 051094.9 kg] predominately of Angus breeding were used to determine the effect of dietary crude protein (CP) level [8.5, 10.7, 12.3 or 14.5%, dry matter (DM) basis; high-moisture-corn-based diets] on visceral mass, cellularity, and protein expression of ATP synthase, Na ' /K ' -ATPase, proliferating cell nuclear antigen (PCNA) and ubiquitin. Steers were on dietary treatment for 28 d. Kidney, liver, and reticulorumen weights (g) increased linearly (PB0.05) with increased dietary CP. Lung weight (g; g kg (1 BW) linearly increased (P B0.05) with increased CP. Ruminal and small intestinal DNA concentration, and the ratios of RNA:DNA and protein:DNA in small intestine were affected quadratically by increased dietary CP (P B0.05). Hepatic ATP synthase expression was affected quadratically with an increase when dietary CP increased from 8.5 to 10.7%, and a decrease when dietary CP increased from 10.7 to 14.5% (PB0.05). Renal ATP synthase expression decreased linearly (PB0.05) and small intestine mucosal Na ' /K ' -ATPase expression increased linearly (P 00.05) with increased CP. These results indicate that increasing dietary CP increases liver, kidney, lung, and rumen masses, and differentially influences expression of proteins influencing energy utilization and efficiency in liver, kidney, and small intestine. . Incidence de la concentration de prote´ines brutes dans l'alimentation sur la masse des visce`res, la cellularite´ainsi que l'expression de l'ATP synthe´tase, de la Na /K -ATPase, du PCNA et de l'ubiquitine dans les prote´ines chez les bouvillons d'engrais. Can. J. Anim. Sci. 89: 493Á 501. Vingt-quatre bouvillons (poids corporel initial 051094,9 kg) essentiellement de race Angus ont servi a`pre´ciser les effets de la quantite´de prote´ines brutes dans les aliments (8,5, 10,7, 12,3 ou 14,5 % de la matie`re se`che; ration a`base de maı¨s a`haute teneur en eau) sur la masse des visce`res, sur la cellularite´, et sur l'expression de l'ATP synthe´tase, de la Na ' / K ' -ATPase, du PCNA (proliferating cell nuclear antigen) et de l'ubiquitine dans les prote´ines. Les bouvillons ont rec¸u leur re´gime pendant 28 jours. Le poids des reins, du foie et du re´ticulorumen (g) augmente de fac¸on line´aire (PB0,05) avec la concentration de prote´ines brutes. Le poids des poumons (g; g par kg de poids corporel) s'accroıˆt line´airement (PB0,05) avec la concentration de prote´ines brutes. La concentration d'ADN dans le rumen et l'intestin greˆle, ainsi que les ratios ARN/ADN et prote´ine/ADN dans l'intestin greˆle sont influence´s de manie`re quadratique par la hausse de la concentration de prote´ines brutes dans la ration (PB0,05). L'expression de l'ATP synthe´tase dans le foie accuse aussi l'influence quadratique de la concentration de prote´i...
The objective of this study was to determine the effect of acetylated peptides on rumen fermentation and nitrogen metabolism in sheep. Six adult Poll × Dorset crossbred sheep, fi tted with permanent rumen and duodenal fi stulas, were used in a replicated 3 × 3 Latin design experiment. Three basic diets, balanced to similar nitrogen intake, were supplemented with 100 g soyabean meal (SBM), 60 g soyabean peptides (SBP) or 80 g acetylated soyabean peptides (ASP), respectively. The crude protein of soyabean peptides and acetylated peptides powder were 66.8 and 51.0%. The degree of acetylation was 88.9%. Soyabean peptides had the highest rumen pH (6.94), followed by ASP and SBM (6.74 and 6.58; P<0.05). Ruminal ammonia was also affected by treatment (8.05 and 10.18 mg/dl for ASP and SBP, respectively; P<0.05). Blood urea nitrogen of the SBM diet showed a higher value compared with SBP and ASP (5.96, 4.14 and 2.90 mmol/l; P<0.05). Apparent nitrogen digestibility of ASP (73.51%) was signifi cantly higher than that of SBM (62.85%; P<0.05).
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