Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/DXS6797.htm. The volume of PCR reaction for each locus was 37.5 µL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of female population genetics and forensic science were analyzed using POWERSTATS program (3). The complete dataset can be accessed at http://www. legalmed.org/dna/DXS6797.htm.
Whole blood obtained by venipuncture from 107 unrelated males of the Han ethnic group in Chengdu, China. DNA was extracted using the Chelex method (1). The reaction volume of PCR was 37.5 μL, containing 2–4 ng human genome DNA, 200 μM each dNTP (Pharmacia, Sweden), 1.5μ Taq polymerase (Promega, Madison, WI), 3.75 μL 10 × buffer, Mg2+ 1.5 mM, 1.6 μg/mL BSA, 0.3 μM each primer. Amplification reactions were carried out in a Perkin Elmer 9600 (Foster City, CA) with pre-denaturing for 2 min at 94°C, followed by 35 cycles of denaturing for 40 s at 94°C, annealing for 40 s at 58°C and extension for 25 s at 72°C. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of population genetics and forensic science were analyzed according to Hou's method (3).
A total 107 EDTA-blood samples was collected from unrelated males of Han population in Chengdu of China. DNA was extracted utilizing the Chelex-100 method (1). The allelic variation at the two Y-STR loci named as DYS544 and DYS587 were analyzed by PCR amplification. Each PCR reaction contained 2-5 ng human genome, 1 × Taq buffer, 1.5 mM MgCl2, 200 μM each dNTP (Pharmacia Biotech, Sweden), 2 U Taq polymerase (Promega Corporation), 0.3 μM each primer, in a total volume of 37.5 μL. PCR amplifications were carried out in a GeneAmp PCR System 9600 (Perkin-Elmer) with pre-denaturing for 2 min at 94°C, followed by 33 cycles of denaturing for 30 s at 94°C, annealing for 60 s at 58°C, and extension for 30 s at 72°C.
Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). Reaction condition of PCR amplification can be accessed at http://www.legalmed.org/ dna/D11S1977.htm. The volume of PCR reaction for each locus was 37.5 µL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system, and visualized by silver staining (2,3). Data of population genetics and forensic science were analyzed using POWERSTATS program (4). The genotype distribution was analyzed for Hardy-Weinberg equilibrium according to Hou's method (5) and no deviation from Hardy-Weinberg equilibrium was observed. The complete data can be accessed at http://www.legalmed.org/dna/D11S1977.htm.
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