Distributions of Allelic Frequencies and Haplotypes of Two Novel Y-Chromosome STR in a Chinese Population

Abstract

Whole blood obtained by venipuncture from 107 unrelated males of the Han ethnic group in Chengdu, China. DNA was extracted using the Chelex method (1). The reaction volume of PCR was 37.5 μL, containing 2–4 ng human genome DNA, 200 μM each dNTP (Pharmacia, Sweden), 1.5μ Taq polymerase (Promega, Madison, WI), 3.75 μL 10 × buffer, Mg2+ 1.5 mM, 1.6 μg/mL BSA, 0.3 μM each primer. Amplification reactions were carried out in a Perkin Elmer 9600 (Foster City, CA) with pre-denaturing for 2 min at 94°C, followed by 35 cycles of denaturing for 40 s at 94°C, annealing for 40 s at 58°C and extension for 25 s at 72°C. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of population genetics and forensic science were analyzed according to Hou's method (3).

Properties of novel and widely studied Y-STR loci in three South African populations

“…In order to select loci from previously published population data, gene diversity values were tabulated for 30 Y-STR loci not included in the minimal haplotype [3,6,7,[10][11][12][13][14][15][16][17]. Loci with gene diversity values consistently above 0.6 were selected.…”

“…Firstly, the single-copy loci of the minimal haplotype were selected primarily as a point of reference against which the performance of other markers could be judged. Secondly, a set of relatively widely studied Y-STR loci were selected on the basis of high gene diversity values reported in a number of populations [3,6,7,[10][11][12][13][14][15][16][17]. The selected loci include DYS439, DYS446, DYS449, DYS452, DYS458, DYS463, DYS635 (Y-GATA-C4) and Y-GATA-A10.…”

“…A repeat nomenclature consistent with International Society of Forensic Genetics (ISFG) guidelines would be (AGAT) n (AGAC) n [26]. For DYS714 the two alleles sequenced had the structure (TTTCT) 13 (CTTCT) 2 (TTTCT) 2 (CTTCT) 2 (TTTCT) 2 , designated allele 21, and (TTTCT) 18 (CTTCT) 2 (TTTCT) 2 (CTTCT) 2 (TTTCT) 2 , designated allele 26. A repeat nomenclature consistent ISFG guidelines would be (TTTCT) n (CTTCT) 2 (TTTCT) 2 (CTTCT) 2 (TTTCT) 2 [26].…”