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Тhe Melanocortin-4 Receptor (MC4R), a G-protein coupled receptor, is implicated in mediating the effect of leptin on food intake and energy balance. A previous candidate gene study reported an association between an MC4R polymorphism (Asp298Asn) and growth and meat productive traits of pigs. The aim of this work was to determine frequencies of the MC4R/Taq I genotypes and alleles in Danish Landrace (LD), Canadian Landrace (LC) and crossbred pigs Danish Landrace × Canadian Landrace (LD × LC) in breeding Cent "Plemzavod Jubilee" (Tyumen) Russia and to estimate their associations with some growth and meat traits. The polymorphism was identified by PCR-RFLP method. The traits studied were: Number of days to 100-kg, Average Daily Gain (ADG), Backfat Thickness (BFT), average daily Feed Intake (FI) and Length of Body (LB). In the all test groups we found a higher frequency of G allele (0.71) compared with the A allele (0.29). The results of present study suggest that the MC4R gene contributes to Days to 100 kg, ADG and BF. The heterozygous genotype AG was favorable in LD breed. The significant effects of-1.43 (LD male) and-2.81(LD female) for Days to 100 kg and of+61.17 (LD male) and +26.3 (LD female) for ADG were calculated. The homozygous genotype GG was favorable in crossbred LD × LC. The significant effects of +74.2 for ADG and-1.5 for BF were calculated. Therefore, the data support a role for the MC4R Asp298Asn polymorphism in the genetic basis of economically important traits in pigs of Russia. Further research is needed to determine the effect of this gene on growth, meat and reproduction traits in pigs of different breeds and crosses.

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One of the areas of biotechnology sunflower is the development and testing of DNA markers of important agronomic traits and in particular markers of resistance to downy mildew. Resistance of 16 Rf-lines of sunflower to the races 330 and 710 of Plasmopara halstedii has been studied. Genotyping of these lines was carried out using 9 STS-markers of three Pl-loci, Pl 5 , Pl 6 and Pl 8 , associated with the resistance of sunflower to downy mildew. Only two out of nine STS-markers, НаР2 and НаР3 (locus Pl 6), allowed us to identify the lines, which demonstrated resistance to the downy mildew under the conditions of artificial infection.

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The paper presents the results of studies of the sequence of the D-loop of mitochondrial DNA of the Volgograd sheep breed. Mitochondrial DNA has a number of unique features that make it possible to effectively use markers based on it in phylogenetic studies of a wide range of organisms.

This paper presents the results on the study of melanocortin-4 receptor (MC4R) gene polymorphisms in sheep of the Karachai breed. AA, AG, and GG genotypes were determined to have allele frequencies of 47%, 37%, and 16%, respectively, indicating that allele A and the homozygous AA genotype occurred the most frequently. Analysis of the meat productivity of sheep, with respect to the MC4R genotype, showed a higher slaughter yield in sheep with the AA genotype compared to those with either the GG or AG genotypes. The variability at these loci provides a basis for controlling the meat productivity of sheep in the Karachai sheep population; therefore, this polymorphism should be monitored and melanocortin-4 receptor (MC4R) gene selection considered in pedigree farms.

Ensuring food security of the state provides for obtaining high yields of agricultural crops and the development of livestock facilities. The aim of the investigation is to develop an accelerated biotechnological preparation of sewage and livestock manure from livestock farms for irrigation of pastures and fertilization of fields for forage crops. Closed-loop systems for products output on pig farms and cattle farms using biotechnology for the preparation of livestock manure for irrigation of fields in the production of forage crops have been developed. The biotechnology of accelerated preparation of sewage and liquid livestock manure is based on the principles of their mechanical separation into fractions with the subsequent preparation of liquid concentrated organic fertilizers using nutraceuticals, which speed up the processes of their preparation in a much shorter time than using traditional technologies. A closed-loop system for products output on sheep and pig farms and cattle farms using biotechnology is proposed. The basis of biotechnology in the processing of solid livestock manure is the principle of accelerated components composting using nutraceuticals. The implementation of accelerated preparation system of sewage and livestock manure for irrigation of pastures and fertilization of fields for forage crops contributed to an increase in their yield up to 20.54%. At the same time, the milk yield per forage cow increased by 8.47-14.20%; average daily weight gain - by 13.30–47.73%.