Breast cancer is the second leading cause of cancer‐related deaths in women. The long noncoding RNA LINC00115 has been reported to be involved in the poor outcome of patients with breast cancer, but the biological function and underlying mechanism remain unclear. Here, we report that LINC00115 expression is increased in triple‐negative breast cancer tissue compared with matched normal tissue, and LINC00115 knockdown suppresses breast cancer cell migration and invasion. Furthermore, we show that LINC00115 directly targets miR‐7 and inhibits its expression. LINC00115 also reduces the expression of KLF4, which is a direct target of miR‐7 and is involved in breast cancer metastasis. Together, our findings suggest that LINC00115 promotes breast cancer metastasis through modulating the expression of miR‐7 and KLF4.

Substantial evidence indicates that circular RNAs (circRNAs) play vital roles in several diseases, especially in cancer development. However, the functions of circRNAs in breast cancer metastasis remain to be investigated. This study aimed to identify the key circRNAs involved in epithelial mesenchymal transition (EMT) of breast cancer and evaluated their molecular function and roles in pathways that may be associated with tumor metastasis. An EMT model was constructed by treating breast cancer cells McF-7 and MdA-MB-231 with transforming growth factor-β1. High-throughput RNA sequencing was used to identify the differentially expressed circRNAs in EMT and blank groups of two cells, and reverse transcription-quantitative PcR was used to validate the expression of circScYL2 in human breast cancer tissues and cells. The effects of circScYL2 on breast cancer cells were explored by transfecting with plasmids and the biological roles were assessed using transwell assays. EMT groups of breast cancer cells exhibited the characteristics of mesenchymal cells. Furthermore, the present study found that 7 circRNAs were significantly upregulated in both the MCF-7 EMT and MdA-MB-231 EMT groups, while 16 circRNAs were significantly downregulated. The current study identified that circScYL2 was downregulated in breast cancer tissues and cell lines, and that circScYL2 overexpression inhibited cell migration and invasion. This study provides expression profiles of circRNAs in EMT groups of breast cancer cells. circScYL2, which is downregulated in breast cancer tissues and cells, may play an important role in breast cancer EMT progression. EMT related circular RNA expression profiles identify circSCYL2 as a novel molecule in breast tumor metastasis

triple-negative breast cancer (tnBc) has a more aggressive phenotype and higher metastasis and recurrence rates than other breast cancer subtypes. tnBc currently lacks a transplantation model that is suitable for clinical simulations of the tumor microenvironment. intraductal injection of tumor cells into the mammary duct could mimic the occurrence and development of breast cancer. Herein, we injected 4T1 cells into the mammary ducts of BALB/C mice to build a preclinical model of tnBc and optimized the related construction method to observe the occurrence and spontaneous metastasis of tumors. We compared the effects of different cell numbers on tumorigenesis rates, times to tumorigenesis, and metastases to determine the optimal number of cells for modelling. We demonstrated that 4T1-MIND model mice injected with 20,000 cells revealed a suitable tumor formation rate and time, thus indicating a potential treatment time window after distant metastasis. We also injected 20,000 cells directly into the breast fat pad or breast duct for parallel comparison. The results still showed that the 4T1-MIND model provides sufficient treatment time for lung metastases in mice and that it is a more reliable model for early tumor development. The 4T1-MIND model requires continuous improvement and optimization. A suitable and optimized model for translational research and studies on the microenvironment in tnBc should be developed.Breast cancer is the most commonly diagnosed cancer type and the leading cause of cancer death among women worldwide 1,2 . Breast ductal carcinoma in situ (DCIS) accounts for 15%-30% of all breast cancer cases 3 . DCIS tends to break through the matrix and develop into invasive ductal carcinoma 4 . Approximately 90% of all breast cancer-related deaths are caused by metastasis 5,6 , the most frequent sites of which are the lung, bone, liver, and brain. Triple-negative breast cancer (TNBC) is remarkably heterogeneous in terms of the tumor microenvironment 7 . Preclinical models that can return the breast tumor microenvironment to its original condition play a pivotal role in studies on the pathogenesis and treatment of breast cancer. However, 23 anticancer drugs failed in clinical trials between 2007 and 2010 8 , partly because the preclinical models used to test them were inappropriate for breast cancer. These findings illustrate that the success of breast cancer research and treatment depends on the experimental animal model used.Experimental animal models of breast cancer have been widely studied. Researchers should choose different animal models based on the needs of their experimental work. Today, the most frequently used breast cancer models include spontaneous breast cancer animal models 9-11 , induced breast cancer animal models 11-15 , allograft experimental animal mammary cancer models [16][17][18][19] , xenograft breast cancer models 20-24 , distant metastasis of breast cancer models 17,25-27 and genetically engineered mouse models (GEMMs) of breast cancer [28][29][30] . Given the Pathological fe...

Background Brain metastasis (BM) is a dreadful complication that significantly impacts the quality of life in breast cancer patients. A key process during brain metastasis is the migration of cancer cells across blood–brain barrier (BBB). However, the role of snoRNAs regulating BBB in BM is still unknown. Methods Here SNORic and GEO databases were used to identify differentially expressed snoRNAs between brain metastatic and non-metastatic breast cancer (BC) tissues. The effects of SNORA71B on the capacities of proliferation, migration, invasion, epithelial-mesenchymal transition (EMT), and BBB invasion of BC cells were evaluated by CCK8, transwell, western blot, and BBB model, respectively. Results SNORA71B was highly expressed in high BM BC tissues and cells compared to low BM BC controls. Survival analysis revealed high expression of SNORA71B was significantly associated with poor PPS and OS in breast cancer patients. ROC curve showed that SNORA71B might act as biomarker for breast cancer. Moreover, SNORA71B significantly promoted proliferation, migration, and invasion of BC cells with different BM abilities. Importantly, SNORA71B promoted the EMT process of low BM BC cells. SNORA71B knockdown inhibited the high BM BC cells across BBB, while EMT activator dramatically abrogated this inhibited effect. Conclusions In conclusion, SNORA71B promotes BC cells across the BBB partly via inducing EMT.

No abstract

No abstract

Abstract. This paper reports an experimental study on the microstructure and corrosion resistance of electro-less Ni-P coatings with increasing content of the rare element cerium (Ce). Surface morphology and the composition of the electro-less Ni-P coatings were studied by scanning electron microscope (SEM), X-ray energy dispersed analysis (EDS) and X-ray diffraction analysis (XRD). Hardness and Adhesive force are researched by a HX-200 Vickers diamond indenter micro-hardness tester. Furthermore, we study the adhesive force by using the Revetest scratch tester. We get the possession of Ce amorphous Ni-P coatings which has excellent properties in anti-corrosion. The effect of the rare element cerium concentration on corrosion resistance of the coatings was evaluated in the groundwater immersion test and porosity test, respectively. The results indicated that added little the rare element cerium into the plating bath increased the phosphorus content of the coatings, decreased the corrosion rates, it also decreases the porosity of the amorphous Ni-P coatings. The lowest corrosion rates of the amorphous Ni-P coatings in groundwater immersion test is 4.1 um·h -1 , at the rare element cerium concentration of 0.12g·L -1.

No abstract