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A novel and sensitive strategy which integrated a DNA cycle device onto magnetic microbeads (MB), amplifying the signal with graphene oxide (GO) and enhancing electrochemiluminescence (ECL) intensity, was developed and was further successfully applied to thrombin detection.

Dysfunctional interactions of amyloid-β (Aβ) with Zn and Cu ions are proved to be related to the etiology of Alzheimer's disease (AD). Disruption of these metal-Aβ interactions using metal chelators holds considerable promise as a therapeutic strategy to combat this incurable disease. Herein, we report that two cyclam derivatives (L1 and L2) are capable of modulating Zn(2+)/Cu(2+)-mediated Aβ40 aggregation, reactive oxygen species (ROS) production, and neurotoxicity. These chelators were found to inhibit the metal-induced Aβ40 aggregation, dissociate metal-Aβ40 aggregates and restore the metal-induced β-sheet structure of Aβ40 to its random coil conformation, as observed by BCA protein assay, thioflavin T fluorescence and circular dichroism spectroscopy. Moreover, preliminary investigation of SH-SY5Y cells indicates that L1 and L2 can diminish the neurotoxicity of metal-Aβ40 species, control metal-Aβ40-triggered ROS production and protect cells against apoptosis. These observations warrant the further investigations of L1 and L2 as potential anti-AD agents.