The rapid and accurate acquisition of nitrogen, phosphorus and potassium nutrient contents in grape leaves is critical for improving grape yields and quality and for industrial development. In this study, crop growth was non-destructively monitored based on unmanned aerial vehicle (UAV) remote sensing technology. Three irrigation levels (W1, W2 and W3) and four fertilization levels (F3, F2, F1 and F0) were set in this study, and drip irrigation fertilization treatments adopted a complete block design. A correlation analysis was conducted using UAV multispectral image data obtained from 2019 to 2021 and the field-measured leaf nitrogen content (LNC), leaf potassium content (LKC) and leaf phosphorus content (LPC) values; from the results, the vegetation indices (VIs) that were sensitive to LNC, LKC and LPC were determined. By combining spectral indices with partial least squares (PLS), random forest (RF), support vector machine (SVM) and extreme learning machine (ELM) machine-learning algorithms, prediction models were established. Finally, the optimal combinations of spectral variables and machine learning models for predicting LNC, LPC and LKC in each grape growth period were determined. The results showed that: (1) there were high demands for nitrogen during the new shoot growth and flowering periods, potassium was the main nutrient absorbed in the fruit expansion period, and phosphorus was the main nutrient absorbed in the veraison and maturity periods; (2) combining multiple spectral variables with the RF, SVM and ELM models could result in improved LNC, LPC and LKC predictions. The optimal prediction model determination coefficient (R2) derived during the new shoot growth period was above 0.65, and that obtained during the other growth periods was above 0.75. The relative root mean square error (RRMSE) of the above models was below 0.20, and the Willmott consistency index (WIA) was above 0.88. In conclusion, UAV multispectral images have good application effects when predicting nutrient contents in grape leaves. This study can provide technical support for accurate vineyard nutrient management using UAV platforms.
Understanding variations in sap flow rates and the environmental factors that influence sap flow is important for exploring grape water consumption patterns and developing reasonable greenhouse irrigation schedules. Three irrigation levels were established in this study: adequate irrigation (W1), moderate deficit irrigation (W2) and deficit irrigation (W3). Grape sap flow estimation models were constructed using partial least squares (PLS) and random forest (RF) algorithms, and the simulation accuracy and stability of these models were evaluated. The results showed that the daily mean sap flow rates in the W2 and W3 treatments were 14.65 and 46.94% lower, respectively, than those in the W1 treatment, indicating that the average daily sap flow rate increased gradually with an increase in the irrigation amount within a certain range. Based on model error and uncertainty analyses, the RF model had better simulation results in the different grape growth stages than the PLS model did. The coefficient of determination and Willmott’s index of agreement for RF model exceeded 0.78 and 0.90, respectively, and this model had smaller root mean square error and d-factor (evaluation index of model uncertainty) values than the PLS model did, indicating that the RF model had higher prediction accuracy and was more stable. The relative importance of the model predictors was determined. Moreover, the RF model more comprehensively reflected the influence of meteorological factors and the moisture content in different soil layers on the sap flow rate than the PLS model did. In summary, the RF model accurately simulated sap flow rates, which is important for greenhouse grape irrigation.
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