Water-insoluble β - ( 1 - 3 ) – D - glucan isolated from the sclerotium of Poria cocos hardly exhibits biological activity. Therefore, it is advantageous to produce a value-added product from Poria cocos. We extracted the β - ( 1 - 3 ) – D - glucan from the sclerotium of Poria cocos and synthesized a carboxymethylated derivative, carboxymethyl-pachyman (CMP). The influences on the degrees of substitution ( DS ) of CMP, for example, volume ratio of ethanol to water, [NaOH]/[MCA] ratio, reaction temperature and reaction time have been examined, respectively. The most favorable conditions for pachyman carboxymethylation are obtained with a [NaOH]/[MCA] ratio of 1.5, at 45°C for 60 minutes with a reaction medium consisting of a ethanol/water 80:20 (v/v) mixture.
Probiotics are of great beneficial functions, especially on gastrointestinal health. Probiotic yoghurt is gaining popularity for it is the main way to supplement probiotics. In this study, a novel probiotic yoghurt was made by adding a freeze-dried aerotolerant Bifidobacterium animailis subsp. lactis Qq08 (Bl Qq08) to traditional yoghurt. Microbial, physical, chemical and flavor changes of the yoghurt during cold storage were analyzed weekly to study the effects of Bifidobacterium addition on the yoghurt quality. Results showed that the addition of Bl Qq08 didn’t significantly affect the cell viabilities in yoghurt which kept above the recommended level. Chemical and flavor characteristics of the probiotic yoghurt kept similar or turned better, indicating that the lyophlized Bl Qq08 was an excellent probiotic bacteria to product yoghurt.
The aim of the present work was to optimize the formula of cryoprotectants for freeze drying process of the previous selectedBifidobacteriumanimailissubsp.lactisQq08 (Bl Qq08). Single factor and orthogonal experiments were conducted to optimize the cryoprotectants formula. The formula of cryoprotectants was optimized as follows: skim milk 15%, lactose 8%, Vc 1.5% and glycerol 3%. In the triplicate lyophilization tests, the average survival rate of Bl Qq08 reached 91.23%.The optimized formula of cryoprotectants was suitable for freeze drying of the Bl Qq08.
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