Ceria nanocubes (NC) modified with increasing concentrations of praseodymium (5, 10, 15, and 20 mol %) have been successfully synthesized by a hydrothermal method. The as-synthesized Pr-modified ceria nanocubes exhibit an enhanced oxidase-like activity on the organic dye TMB within a wide range of concentrations and durations. The oxidase activity increases with increasing Pr amounts in Pr-modified ceria nanocubes within the investigated concentration range. Meanwhile, these Pr-modified ceria nanocubes also show higher reducibility than pure ceria nanocubes. The kinetics of their oxidase mimetic activity is fitted with the Michaelis-Menten equation. A mechanism has been proposed on how the Pr incorporation could affect the energy level of the bands in ceria and hence facilitate the TMB oxidation reaction. The presence of Pr species on the surface also contributes to the increasing activity of the Pr-modified ceria nanocubes present higher oxidase activity than pure ceria nanocubes.
Quantitative
detection and characterization of antigen-specific
T cells are crucial to our understanding of immune responses as well
as the development of new immunotherapies. Herein, we report a spatiotemporally
resolved method for the detection and quantification of cell–cell
interactions via Photocatalytic proXimity CELl Labeling (PhoXCELL). The biocompatible photosensitizer dibromofluorescein
(DBF) was leveraged and optimized as a nongenetic alternative of enzymatic
approaches for efficient generation of singlet oxygen upon photoirradiation
(520 nm) on the cell surface, which allowed the subsequent labeling
of nearby oxidized proteins with primary aliphatic amine-based probes.
We demonstrated that DBF-functionalized dendritic cells (DCs) could
spatiotemporally label interacting T cells in immune synapses via
rapid photoirradiation with quantitatively discriminated interaction
strength, which revealed distinct gene signatures for T cells that
strongly interact with antigen-pulsed DCs. Furthermore, we employed
PhoXCELL to simultaneously detect tumor antigen-specific CD8+ as well as CD4+ T cells from tumor-infiltrating lymphocytes
and draining lymph nodes in murine tumor models, enabling PhoXCELL
as a powerful platform to identify antigen-specific T cells in T cell
receptor (TCR)-relevant personal immunotherapy.
Tumor-targeted and stimuli-activatable nanosensitizers are highly desirable for cancer theranostics. However, designing smart nanosensitizers with multiple imaging signals and synergistic therapeutic activities switched on is challenging. Herein, we report tumortargeted and redox-activatable nanosensitizers (1-NPs) for sono-photodynamic immunotherapy of tumors by molecular co-assembly and redox-controlled disassembly. 1-NPs show a high longitudinal relaxivity (r 1 = 18.7 � 0.3 mM À 1 s À 1 ), but "off" dual fluorescence (FL) emission (at 547 and 672 nm), "off" sono-photodynamic therapy and indoleamine 2,3-dioxygenase 1 (IDO1) inhibition activities. Upon reduction by glutathione (GSH), 1-NPs rapidly disassemble and remotely release small molecules 2-Gd, Zn-PPA-SH and NLG919, concurrently switching on (1) dual FL emission, (2) sonophotodynamic therapy and (3) IDO1 inhibition activities. After systemic injection, 1-NPs are effective for bimodal FL and magnetic resonance (MR) imagingguided sono-photodynamic immunotherapy of orthotropic breast and brain tumors in mice under combined ultrasound (US) and 671-nm laser irradiation.
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