Background Fragility fracture is one of the most serious consequences of female aging, which can increase the risk of death. Therefore, paying attention to the pathogenesis of postmenopausal osteoporosis (PMOP) is very important for elderly women. Methods and materials Forty 12-week-old female rats were divided into two groups including the ovariectomy (OVX) group and the control group. Four rats in each group were selected at 1, 4, 8, 12 and 16 weeks after operation. Vertebral bones and femurs were dissected completely for micro-Computed Tomography (micro-CT) scanning, biological modulus detection and histomorphological observation. Results In OVX group, bone volume/total volume (BV/TV), bone trabecular connection density (Conn.D) and trabecular bone number (Tb.N) decreased significantly with time (P < 0.05). The elastic modulus of femur in OVX group was lower than that in control group, but there was no significant difference between them (P > 0.05). Over time, the tartrate resistant acid phosphatase (TRAP), osteocalcin (BGP), type I procollagen amino terminal propeptide (PINP) and type I collagen carboxy terminal peptide (CTX-I) in OVX group increased significantly (P < 0.05). The micrographs of the OVX group showed sparse loss of the trabecular interconnectivity and widening intertrabecular spaces with time. Conclusion The bone loss patterns of vertebral body and femur were different in the early stage of estrogen deficiency. The bone turnover rate of OVX rats increased, however the changes of biomechanical properties weren’t obvious.
Background: Fragility fracture is one of the most serious consequences of female aging, which can increase the risk of death. Therefore, paying attention to the pathogenesis of PMOP is very important for elderly women.Methods and materials: Forty 12-week-old female rats were divided into two groups including the ovariectomy group and the control group. Four rats in each group were selected at 1, 4, 8, 12 and 16 weeks after operation. Vertebral bodies and femurs were dissected completely for micro-CT scanning, biological modulus detection and histomorphological observation.Results: In OVX group, BV/TV, Conn.D and Tb.N decreased significantly with time (P<0.05). The elastic modulus of femur in OVX group was lower than that in control group, but there was no significant difference between them (P>0.05). Over time, the TRAP, BGP, PINP and CTX-I in OVX group increased significantly (P<0.05). The micrographs of the OVX group showed sparse loss of the trabecular interconnectivity and widening intertrabecular spaces over time. Conclusion: The bone turnover rate of OVX rats in the early stage of estrogen deficiency increased. The bone loss patterns of vertebral body and femur were different, and the changes of biomechanical properties weren’t obvious.
Background: With the application of material decomposition and monoenergetic imaging reconstruction technology of spectral CT in the musculoskeletal system, the purpose of this study was to explore the quantitative changes of L5 vertebral body in ovariectomized rats by spectral CT Gemstone Spectral Imaging (GSI) and dual energy X-ray absorption (DXA).Methods: Spectral CT GSI and DXA scans of the forty rats were conducted at 0 (baseline), 4, 8 and 12 weeks after operation. Hydroxyapatite (HAP)/fat value, 74KeV monoenergetic CT value and bone mineral density (BMD) of L5 vertebral body were measured. After excessive anesthesia, L5 vertebral body was taken for histological observation. Results: There was significant difference in HAP(fat) value between groups at 4, 8 and 12 weeks after operation (P<0.05), and the significant difference in 74KeV monoenergetic CT value and BMD at 8 and 12 weeks after operation (P<0.05). There was also a positive correlation between 74KeV monoenergetic CT value and BMD of L5 vertebral body in OVX group. From 4 weeks after operation, the L5 of the OVX group showed sparse loss of the trabecular interconnectivity, thinning of the trabeculae and widened intertrabecular spaces.Conclusion: Spectral CT material decomposition technology can be used to detect the bone state of vertebral body in the early stage of estrogen deficiency and has the better sensitivity than the BMD.
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