Increasing human activities are altering marine ecosystems, which may have ramifications for predator feeding ecology and, thus, the degree of contaminant exposure. We conducted the first investigation of spatiotemporal trends for nine alternative halogenated flame retardants (AHFRs) and their relations with dietary variations in 128 humpback dolphins that were stranded along the northern South China Sea during 2003–2020. We detected the highest levels of seven major AHFRs in humpback dolphins compared with the results reported in cetaceans globally, indicating high AHFR contamination in coastal regions of South China. Dolphins that were stranded near urban regions generally contained higher AHFR concentrations than those that were stranded near rural areas, mirroring the environmental trends of AHFRs occurring in this area. Model-generated diet estimates suggested that humpback dolphins have reduced their consumption of high trophic-level prey in recent years, likely attributable to overfishing-induced prey decline in this region. After adjusting AHFR concentrations due to diet changes, the temporal trends of AHFR contamination in humpback dolphins were only slightly altered. Our results suggest that increasing discharges of AHFRs into the South China Sea during the 2000s and 2010s may have had a greater influence on AHFR trends in humpback dolphins than dietary shifts.

Marine mammals often accumulate high levels of environmental contaminants, even those that are globally regulated regarding usage, raising concerns about their health status. Here, we conducted the first investigation of tissue distribution, spatiotemporal trends, and potential risks of six organotin compounds (OTs) in Indo-Pacific humpback dolphins (n = 101) from the northern South China Sea during 2003–2021. We detected the highest level of hepatic triphenyltin in these humpback dolphins compared with the results reported in cetaceans globally, and the liver accumulated the highest OT concentrations than other analyzed tissues. Despite the downward trend of butyltins in humpback dolphins after the global ban on the use of OTs as antifouling paints, levels of phenyltins have continued to increase over the past 20 years, suggesting that the other applications of phenyltins in South China remain prevalent. In vitro and in vivo analyses revealed that tissue-relevant doses of OTs could induce agonistic effects on the dolphin peroxisome proliferator-activated receptor γ as a master regulator of lipid homeostasis and altered the dolphin fatty acid profiles. Our results highlight the lipid-disrupting effects of current OT exposure in humpback dolphins and emphasize the need for further efforts to eliminate OT contamination in South China.

The potential risks of per-and polyfluoroalkyl substance (PFAS) accumulation in nearshore dolphins are not well understood. Here, transcriptional activities of 12 PFAS on peroxisome proliferator-activated receptors (PPAR-α, -β/δ, and -γ) in Indo-Pacific humpback dolphins (Sousa chinensis) were evaluated. All PFAS activated scPPAR-α in a dose-dependent manner. PFHpA exhibited the highest induction equivalency factors (IEFs). The order of IEFs for other PFAS was as follows: PFOA > PFNA > PFHxA > PFPeA > PFHxS > PFBA > PFOS > PFBuS ≈ PFDA ≫ PFUnDA and PFDoDA (not activated). The total induction equivalents (∑IEQs, 5537 ng/g wet weight) indicated that more attention should be paid to investigating contamination levels in dolphins, especially in PFOS (82.8% contribution to the ∑IEQs). The scPPAR-β/δ and -γ were not affected by any PFAS, except for PFOS, PFNA, and PFDA. Furthermore, PFNA and PFDA could induce higher PPARβ/δ and PPAR-γ-mediated transcriptional activities than PFOA. Compared to human beings, PFAS might be more potent PPAR-α activators in humpback dolphins, suggesting that the dolphins may be more susceptible to the adverse effects of PFAS. Our results may be instructive for understanding the impacts of PFAS on marine mammal health due to the identical PPAR ligand-binding domain.

No abstract