Grass carp reovirus (GCRV) seriously affects the grass carp farming, as the causative agent of grass carp hemorrhagic disease. In this study, we found the GCRV 096 NS38 protein contained one conservative PXXP motif similar to σNS of avian reovirus, with very low similarity rates with its homologous GCRV proteins of different genotypes. Furthermore, the soluble GCRV 096 NS38 protein was expressed in Escherichia coli, antiserum was raised and characterised. Addition of purified NS38 protein to culture media of Ctenopharyngodon idella kidney (CIK) cells clearly inhibited replication of GCRV 096 (genotype I); however, replication of GCRV GD108 (genotype Ш) was not significantly affected in CIK cells under the same conditions. It was not possible to induce cross-protection among GCRVs of different genotypes. GCRV multi-genotype should be considered and the NS38 protein was a promising candidate for developing therapies against grass carp hemorrhagic disease. Additionally, bioinformatics analysis suggested that the NS38 protein may be involved in viral genome replication.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.