This study aimed to examine selenite uptake in Medicago sativa L. Potted alfalfa plants were grown in sterilized quartz sand and exposed to 1 and 10 μM selenite for 21 days.Thirty-day-old seedlings and excised roots were used to determine the mechanism of selenite uptake in alfalfa. The following patterns of Selenium (Se) distribution in roots were as follows: lateral roots > taproots and subcellular fraction (I) > fraction (III) > fraction (II). With increasing pH, Se uptake in roots decreased markedly. The dosedependent Se net uptake at pH 5.5 followed the Michaelis-Menten kinetics. Addition of phosphate decreased Se uptake in the excised roots (P < 0.05), whereas a deficiency in phosphate increased Se uptake (P < 0.05). The results indicate that alfalfa mainly absorbed selenium through lateral roots. Selenite uptake by alfalfa root depended on pH, with the preferential form absorption of as H 2 SeO 3 > HSeO 3 1À > SeO 3 2À . A portion of selenite was absorbed actively and may be mediated by phosphate transporter.
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