To identify the accurate gas volume to carry liquid in horizontal gas well, this paper simulates the gas-liquid two-phase flow in a single pipe on the platform of multiphase pipe flow. Through indoor experiments on gas-liquid two-phase flow, it is confirmed that the inclined section (inclined angle: 45°~ 60°) is mostly likely to suffer from liquid loading in horizontal gas well. Then, a new critical liquid-carrying model was built after fitting the liquid holdup calculation formula at different inclined angles, considering the actual liquid-carrying phenomenon. The proposed model was proved accurate enough for engineering application through verification by indoor experiments.
Our study investigates whether miR-265 regulates the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into alveolar type II epithelial cells (ATII) through TGF-β1 and promotes lung injury repair in rats with sepsis, thereby inhibiting sepsis progression.
25 patients with sepsis admitted to the Respiratory and Critical Care Medicine Department of the hospital and 17 normal controls were included. TGF-β1 level was measured by ELISA. miR-265 level was measured by qRT-PCR and AT II-related genes and proteins expression was analyzed
by western blot and qRT-PCR. miR-265 expression was significantly higher in sepsis patients than normal group. Progenitor BMSCs were long and shuttle-shaped after 1 and 3 days of growth. Cultured MSCs had low expression of the negative antigen CD34 (4.32%) and high expression of the positive
antigen CD44 (99.87%). TGF-β1 level was significantly increased with longer induction time, while miR-265 expression was significantly decreased in cell culture medium. miR-265 interference significantly decreased TGF-β1 expression. In conclusion, miR-265 inhibits BMSC
differentiation to AT II via regulation of TGF-β1, thereby inhibiting sepsis progression.
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