Background: Heterosis has been extensively utilized in different crops and made a significant contribution to global food security. Genetic distance (GD) is one of the valuable criteria for selecting parents in hybrid breeding. The objectives of this study were to estimate the GD between parents using both simple sequence repeat (SSR) markers and single nucleotide polymorphism (SNP) markers and to investigate the efficiency of the prediction of hybrid performance based on GD. The experiment comprised of four male parents, 282 female parents and 1128 F1, derived from NCII mating scheme. The hybrids, their parents with two check cultivars were evaluated for two years. Performance of F1, mid-parent heterosis (MPH), and best parent heterosis (BPH) were evaluated for ten agronomic and fiber quality traits. Results: Heterosis was observed in all hybrids and, the traits like plant height, boll number, boll weight and lint percentage exhibited higher heterosis than the fiber quality traits. Correlations were significant between parental and F1 performances. The F1 performances between three hybrid sets (Elite×Elite, Exotic×Elite, and Historic×Elite) showed significant differences in eight traits. The correlation of the GD assessed by both SSR and SNP markers was significantly positive. The cluster analysis based on GD results estimated using SNP showed that all the female parents divided into five groups and the F1 performance between these five groups showed significant differences in seven traits. The correlation between GD and F1 performance, MPH and BPH were significant for lint percentage and micronaire. Conclusions: Our results suggested that GD between parents could be helpful in heterosis prediction for certain traits. This study reveals that molecular marker analysis can serve as a basis for assigning germplasm into heterotic groups and to provide guidelines for parental selection in hybrid cotton breeding.
Background: Cotton stem trichomes and seed fibers are each single celled structures formed by protrusions of epidermal cells, and were found sharing the overlapping molecular mechanism . Compared with fibers, cotton stem trichomes are more easily observed, but the molecular mechanisms underlying their development are still poorly understood. Results: In this study, Gossypium hirsutum (Gh ) and G . barbadense ( Gb ) were found to differ greatly in percentages of varieties/accessions with glabrous stems and in trichome density, length, and number per trichopore. Gh varieties normally had long singular and clustered trichomes, while Gb varieties had short clustered trichomes. Genetic mapping using five F2 populations from crosses between glabrous varieties and those with different types of stem trichomes revealed that much variation among stem trichome phenotypes could be accounted for by different combinations of genes/alleles on Chr.06 and Chr.24. The twenty six F1 generations from crosses between varieties with different types of trichomes had varied phenotypes, further suggesting that the trichomes of tetraploid cotton were controlled by different genes/alleles. Compared to modern varieties, a greater proportion of Gh wild accessions were glabrous or had shorter and denser trichomes; whereas a smaller proportion of Gb primitive accessions had glabrous stems. A close correlation between fuzz fiber number and stem trichome density was observed in both Gh and Gb primitive accessions and modern varieties. Conclusion: Based on these findings, we hypothesize that stem trichomes evolved in parallel with seed fibers during the domestication of cultivated tetraploid cotton. In addition, the current results illustrated that stem trichome can be used as a morphological index of fiber quality in cotton conventional breeding.
Background: Heterosis has been extensively utilized in different crops and made a significant contribution to global food security. Genetic distance (GD) is one of the valuable criteria for selecting parents in hybrid breeding. The objectives of this study were to estimate the GD between parents using both simple sequence repeat (SSR) markers and single nucleotide polymorphism (SNP) markers and to investigate the efficiency of the prediction of hybrid performance based on GD. The experiment comprised of four male parents, 282 female parents and 1128 F1, derived from NCII mating scheme. The hybrids, their parents and two check cultivars were evaluated for two years. Performance of F1, mid-parent heterosis (MPH), and best parent heterosis (BPH) were evaluated for ten agronomic and fiber quality traits, including plant height, boll weight, boll number, lint percentage, fiber length, fiber strength, fiber uniformity, fiber elongation ratio, micronaire, and spinning consistent index. Results: Heterosis was observed in all hybrids and, the traits like plant height, boll number, boll weight and lint percentage exhibited higher heterosis than the fiber quality traits. Correlations were significant between parental and F1 performances. The F1 performances between three hybrid sets (Elite×Elite, Exotic×Elite, and Historic×Elite) showed significant differences in eight traits, including boll number, lint percentage, fiber length, fiber strength, fiber uniformity, fiber elongation ratio, micronaire, and spinning consistent index. The correlation of the GD assessed by both SSR and SNP markers was significantly positive. The cluster analysis based on GD results estimated using SNP showed that all the female parents divided into five groups and the F1 performance between these five groups showed significant differences in four traits, including lint percentage, micronaire, fiber strength, and fiber elongation ratio. The correlation between GD and F1 performance, MPH and BPH were significant for lint percentage and micronaire. Conclusions: Our results suggested that GD between parents could be helpful in heterosis prediction for certain traits. This study reveals that molecular marker analysis can serve as a basis for assigning germplasm into heterotic groups and to provide guidelines for parental selection in hybrid cotton breeding.
Background: Upland cotton (Gossypium hirsutum) accounts for more than 90% of the annual world cotton output because of its high yield potential. However, yield and fiber quality traits often show negative correlations. We constructed four F2 populations of unland cotton, using two normal lines (4133B and SGK9708) with high yield potential but moderate fiber quality and two introgression lines (Suyuan04-3 and J02-247) with superior fiber quality, and used them to investigate the genetic basis underlying complex traits such as yield and fiber quality in upland cotton. We also phenotyped eight agronomic and economic traits and mapped quantitative trait loci (QTLs). Results: Extensive phenotype variations and transgressive segregation were found across the segregation populations. We constructed four genetic maps of 585.97 cM, 752.45 cM, 752.45 cM and 1 163.66 cM, one for each of the four F2 populations.. Fifty QTLs were identified across the four populations (7 for plant height, 27 for fiber quality and 16 for yield). The same QTLs were identified in different populations, including qBW4 and qBW2, which were linked to a common simple sequence repeat (SSR) marker, NAU1255. A QTL cluster containing eight QTLs for six different traits was characterized on linkage group 9 of the 4133B×Suyuan04-3 population. Conclusions: These findings will provide insights into the genetic basis of simultaneous improvement of yield and fiber quality in upland cotton breeding.
Background: Upland cotton (Gossypium hirsutum) accounts for more than 90% of annual world cotton output due to its high yield potential. However, yield traits and fiber quality traits exhibit negative correlations in most cases. Here, we constructed four F2 populations, using two normal lines and two introgression lines, for simultaneously detection the genetic basis underlying complex traits such as yield and fiber quality in upland cotton. Subsequently, the phenotyping of 8 agronomic and economic traits along with quantitative trait loci (QTL) mapping was implemented. Results: Extensive phenotype variations and transgressive segregation were found across segregation populations. Four genetic maps were constructed with the length of 585.97cM, 752.45cM, 752.45cM and 1163.66cM. The mapping resulted in the identification 50 QTLs (27 were for fiber quality traits and 16 for yield traits) across four populations. Multiple QTLs having the common maker, such as qBW4 and qBW2, or residing in the same QTL cluster, such as qLP9 and qFL9-1, were prioritized for further research. Conclusions: These findings will provide insight into the genetic basis of simultaneous improvement of yield and fiber quality in upland cotton breeding.
Background: Upland cotton (Gossypium hirsutum) is one of the important cash crops. Therefore, improving fiber quality of upland cotton, is one of the current important research directions. Cotton fibers are single-celled extensions of the seed epidermis, which is important pattern material of research cytoskeleton. Tubulin genes act an important role in the synthesis of the microtubules (MT), which is core element of the cytoskeleton. Some tubulin genes in cotton ovule and fiber development are were reported, while the systematic study of its family genes has not yet in cotton. Therefore, identification and expression analyses of G. hirsutum tubulin genes provide pivotal targets for molecular manipulation in cotton breeding.Result: In this study, we investigated all tubulin genes from seven varies plant species, and identified 98 tubulin genes in G. hirsutum. Phylogenetic analysis showed that tubulin family genes were classified into three subfamilies. The protein motifs and gene structure of α, β-tubulin genes are more conservative comparing with γ-tubulin genes. Most tubulin genes are located at the proximate ends of the chromosomes. Spatiotemporal expression pattern by transcriptome and qRT-PCR analysis revealed that 12 α-tubulin and 7 β-tubulin genes are specific expression in different stages of fiber development. However, Gh.A03G027200, Gh.D03G169300 and Gh.A11G258900 had clear express pattern at distinct stages of fiber development in J02508 and ZRI015.Conclusion: In this study, evolutionary analysis showed that the tubulin genes were divided into 3 clades. The genetic structures and molecular functions were highly conserved in different plants. Three candidate genes of Gh.A03G027200, Gh.D03G169300 and Gh.A11G258900 may play key roles during length and strength in fiber development with the expression analysis at different organizations.
Background Cotton is known for its fiber and it is grown in tropical and sub-tropical areas of the world. It has a significant role in GDP of Pakistan. Therefore, present two years research was conducted to estimate heritability and association among various yield contributing parameters of cotton. The selected genotypes of cotton were hybridized in green house of the department. The F 0 cotton seed along with parents were planted in the field conditions during May, 2018. The sowing of this experiment was completed in three replications followed by RCBD. The data was recorded at maturity for various agronomic traits including plant height, number of bolls per plant, number of sympodial branches per plant, seed cotton yield, boll weight, seed index, ginning out turn, fiber length, fiber strength, and fiber fineness. Level of significance of data was computed by ANOVA to assess the difference among cotton genotypes which was used for estimation of heritability and correlation analysis among the related traits. Results Association analysis revealed that seed cotton yield had significant positive relationship with plant height, number of bolls per plant, number of sympodial branches per plant, ginning out turn, staple length and fiber strength. Staple length and fiber strength were negatively linked with each other. Estimates of heritability were high for all observed traits except number of sympodial branches per plant and boll weight. Conclusion The parent IUB-222 was found best for plant height, number of bolls per plant, boll weight, ginning out turn, seed cotton yield and seed index. NIAB-414 and VH-367 were identified best parents for fiber length, strength and fineness. Among crosses NIAB-414 × IUB-222 was best for number of bolls per plant, seed index, seed cotton yield and fiber fineness. Whereas, cross NIAB-414 × CIM-632 was good for plant height. The combination of A555 × CIM-632 was best for number of sympodial branches per plant, boll weight, fiber length and strength. VH-367 × CIM-632 proved best for ginning out turn. The correlation results from this study would be helpful to breed cotton cultivars with good yield and quality characters. Broad sense heritability was high for all of parameters which provides the strong evidence that selection in early generations can improve the performance of these traits.
Background: Cotton stem trichomes and seed fibers are each single celled structures formed by protrusions of epidermal cells, and were found sharing the overlapping molecular mechanism. Compared with fibers, cotton stem trichomes are more easily observed, but the molecular mechanisms underlying their development are still poorly understood.Results: In this study, Gossypium hirsutum ( Gh ) and G. barbadense ( Gb ) were found to differ greatly in percentages of varieties/accessions with glabrous stems and in trichome density, length, and number per trichopore. Gh varieties normally had long singular and clustered trichomes, while Gb varieties had short clustered trichomes. Genetic mapping using five F2 populations from crosses between glabrous varieties and those with different types of stem trichomes revealed that much variation among stem trichome phenotypes could be accounted for by different combinations of genes/alleles on Chr. 06 and Chr. 24. The twenty six F1 generations from crosses between varieties with different types of trichomes had varied phenotypes, further suggesting that the trichomes of tetraploid cotton were controlled by different genes/alleles. Compared to modern varieties, a greater proportion of Gh wild accessions were glabrous or had shorter and denser trichomes; whereas a smaller proportion of Gb primitive accessions had glabrous stems. A close correlation between fuzz fiber number and stem trichome density was observed in both Gh and Gb primitive accessions and modern varieties. Conclusion: Based on these findings, we hypothesize that stem trichomes evolved in parallel with seed fibers during the domestication of cultivated tetraploid cotton. In addition, the current results illustrated that stem trichome can be used as a morphological index of fiber quality in cotton conventional breeding.
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