Recently, human hair keratin has been widely studied and applied in clinical fields due to its good histocompatibility, biocompatibility, and biodegradability. However, the regenerated keratin from human hair cannot be electrospun alone because of its low molecular weight. Herein, gamma polyglutamic acid (γ-PGA) was first selected to fabricate smooth and uniform γ-PGA/keratin composite scaffolds with excellent biocompatibility and biodegradability by electrospinning technology and a chemical cross-linking method in this study. The effect of electrospinning parameters on the structure and morphology, the mechanism of chemical cross-linking, biocompatibility in vitro cell culture experiments, and biodegradability in phosphate-buffered saline buffer solution and trypsin solution of the γ-PGA/keratin electrospun nanofibrous scaffolds (ENS) was studied. The results show that the cross-linked γ-PGA/keratin ENSs had excellent water stability and biodegradability. The γ-PGA/keratin ENSs showed better biocompatibility in promoting cell adhesion and cell growth compared with the γ-PGA ENSs. It is expected that γ-PGA/keratin ENSs will be easily and significantly used in tissue engineering to repair or regenerate materials.
Waterproof and moisture permeable composite fabric with electrospun nanofibrous membrane has been widely used, because of its high specific surface area, high porosity, small pore size, uniform pore distribution and light weight. However, there are fewer studies on fabricating waterproof and permeable composite fabric with optimal polyvinylidene fluoride/polyvinylidene fluoride hexafluoropropylene electrospun nanofibrous membrane by multi-needle cross-electrospinning technology and hot-press process. In this study, excellent waterproof and moisture permeable polyvinylidene fluoride/polyvinylidene fluoride hexafluoropropylene electrospun nanofibrous membranes with the optimal process conditions were prepared through central combination design with Design Expert 8.0.5 and experimental validation. The composite fabrics with three different adhesives were prepared. The contact angle, waterproof and moisture permeability, and mechanical properties of composite fabrics were tested. The results showed that CF-1 had better performance with high hydrostatic pressure of 9840 mmH2O, strong moisture permeability of 10,280 g · m−2 · d−1, and excellent peel strength and tensile tenacity. It was of great significance to realize the industrial production of waterproof and breathable composite fabric with electrospun nanofibrous membrane by multi-needle cross-electrospinning technology and hot-press process.
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