Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and methods: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87, 18.75, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. S AB were 100%, and inter-groups similarity coefficients, i.e. S AB were less than 30%. Conclusions: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.
Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and Methods:Positive rates and genotypic profiles of Candida albicansstrains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNApolymerase chain reactions were used to sequence the DNA of these strains, and then their geneticsimilarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence AnalysesSoftware. Results:The prevalence of C. albicansstrains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SABwere 100%, and inter-groups similarity coefficients, i.e. SABwere less than 30%. Conclusions:The genotypic results of C. albicansin OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicansin OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.
Background: The aim of this study wasto investigate the prevalence and genotypic profiles of Candida albicansinpatients with oral lichen planus (OLP). Materials and Methods:Positive rates and genotypic profiles of Candida albicansstrains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNApolymerase chain reactions were used to sequence the DNA of these strains, and then their geneticsimilarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence AnalysesSoftware. Results:The prevalence of C. albicansstrains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SABwere 100%, and inter-groups similarity coefficients, i.e. SABwere less than 30%. Conclusions:The genotypic results of C. albicansin OLPrevealedan endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicansin OLP, with theetiologic sense contributing to a more proper recognition on the pathogenesis,development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.
Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and Methods: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SAB were 100%, and inter-groups similarity coefficients, i.e. SAB were less than 30%. Conclusions: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.
Objectives:This study aimed to investigate the prevalence and genotypic profiles of Candida albicans from patients with oral lichen planus (OLP). Materials andMethods:Genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reaction were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results:The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients S AB were 100%, and inter-groups similarity coefficients S AB were less than 30%. Conclusions:The genotypic evidence of C. albicans in OLP might inferred an endogenous infection and some etiologic sense contributing to professional recognition on the development and progression of OLP for more suitable diagnose and treatment.
Sepsis is a high mortality and great harm systemic inflammatory response syndrome caused by infection. lncRNAs are potential prognostic marker and therapeutic target. Therefore, we expect to screen and analyze lncRNAs with potential prognostic markers in sepsis. We obtained 2310 differentially expressed (DE) lncRNAs and 7310 DEmRNAs by transcriptome sequencing. Then the immune-related lncRNA-mRNA regulatory network, which contains 14 core lncRNAs, was constructed by functional enrichment and Pearson correlation analysis. The results of immune infiltration, gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) further confirmed the correlation between 14 key lncRNAs and immune cells. Subsequently, the key immune-related lncRNA PRKCQ-AS1 was identified by weighted gene co-expression network analysis (WGCNA). RT-qPCR showed that PRKCQ-AS1 was up-regulated in clinical samples and sepsis model cells (LPS-induced HUVECs). Kaplan-Meier (KM), receiver operator characteristic (ROC), Cox regression analysis and nomogram confirmed that PRKCQ-AS1 was an independent prognostic factor in sepsis patients. Immune correlation analysis showed that PRKCQ-AS1 was involved in the immune response and inflammatory process of sepsis. Cell function assay confirmed that PRKCQ-AS1 could inhibit the LPS-induced sepsis model cells viability and promote cell apoptosis, inflammatory damage and oxidative stress. In conclusion, we constructed immune-related lncRNA-mRNA regulatory networks in the progression of sepsis and analyzed the role of PRKCQ-AS1 in the prognosis and progression of sepsis. It is confirmed that PRKCQ-AS1 is an important prognostic factor affecting the progression of sepsis and is involved in immune response.
Mandibular osteomyelitis in a patient with psoriasis is an uncommonly clinical manifestation while there is an increasing number of reports and studies on involvements of stomatology in psoriasis, especially the death of a patient via or not via Allogeneic bone marrow transplantation has never been reported. To review the management and possible mechanisms in pathogenesis and treatment of psoriasis, as well as the relative involvements between stomatology and psoriasis the typical case with pictures and files is reviewed and literature is collected.Wekeepthe knowledge that psoriasis is either a primary keratinocyte disorder or an immunocyte-mediated chronic skin inflammatory disease while bone marrow is under suspected for immunopathogenesis. More association of stomatologic conditions with psoriasis is emerging. Conclusively, allogeneic BMT and new knowledge are worth to be stressed by both stomatological and dermatological doctors. Further insights of this kind of auto immunologic disease are under its developing. He et al.; JAMMR, 31(4): 1-6, 2019; Article no.JAMMR.52565 2 Case Study
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.