Abnormal physiological
levels of sialic acid (SA) could be used
to diagnosis cancer progression stages. In this work, we describe
an enzyme-assist-interference-free strategy for Raman selective determination
of SA in serum. First, we assemble gold nanoparticles (Au NPs) onto
the indium tin oxide glass (ITO) to construct an ITO/Au two-dimension
substrate. Through modification of 4-mercaptoboric acid (4-MPBA) onto
the surface of ITO/Au, the SA response plate is prepared due to the
reversible esterification bond. In this strategy, a sandwich structure
is rationally designed as ITO/Au/4-MPBA/SA/4-MPBA/Au to enhance the
Raman scattering. The Raman detection linear concentration of SA ranged
from 2.5 × 10–7 to 1.5 × 10–6 M, and a limit of detection about 1.2 × 10–7 M could be achieved. Considering the presence of glucose (Glu) in
physiological fluid, we introduce glucose oxidase to remove the interference
from Glu and realize the accurate determination of SA. The proposed
novel Raman rapid method provides an ultrasensitive and interference-free
protocol for the early diagnosis of cancer.
Abuse of illicit drugs has become a major issue of global concern. As a synthetic amphetamine analog, 3,4-Methylene Dioxy Amphetamine (MDA) causes serotonergic neurotoxicity, posing a serious risk to human health. In this work, a two-dimensional substrate of ITO/Au is fabricated by transferring Au nanoparticle film onto indium–tin oxide glass (ITO). By magnetic inducing assembly of Fe3O4@Au onto ITO/Au, a sandwich-based, surface-enhanced Raman scattering (SERS) detection strategy is designed. Through the use of an external magnet, the MDA is retained in the region of hot spots formed between Fe3O4@Au and ITO/Au; as a result, the SERS sensitivity for MDA is superior compared to other methods, lowering the limit of detection (LOD) to 0.0685 ng/mL and attaining a corresponding linear dynamic detection range of 5–105 ng/mL. As an actual application, this magnetically improved SERS sensing strategy is successfully applied to distinguish MDA in urine at trace level, which is beneficial to clinical and forensic monitors.
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