The aim of this study was to increase the Coenzyme Q10 (CoQ10) yield from Rhodobacter sphaeroides via genetic engineering pathway. CoQ10 plays important roles in many biological processes and has been proven to be effective in the treatment of many diseases. In the present study, the ubiG gene located in CoQ10 biosynthesis pathway was effectively overexpressed in Rb. sphaeroides to increase CoQ10 production. The growth of host cells was slightly influenced by overexpressing ubiG. The crude CoQ10 production was enhanced by 58.31% compared to that from the control. The ubiG mRNA level was significantly increased compared to the wild type harboring empty vector as measured by qRT-PCR. Moreover, the crude CoQ10 exhibited strong anti-oxidant activity as measured in vivo by zone of inhibition assay.
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