Fibroblast growth factor receptors (FGFrs) have been implicated in the malignant transformation and chemoresistance of epithelial ovarian cancer; however, the underlying molecular mechanisms are poorly understood. increased sialyltransferase activity that enhances protein sialylation is an important post-translational process promoting cancer progression and malignancy. in the present study, α2,6-sialyltransferase (ST6Gal-i) overexpression or knockdown cell lines were developed, and FGFr1 was examined to understand the effect of sialylation on migration and drug resistance, and the underlying mechanisms. it was identified that cells with ST6Gal-I overexpression had increased cell viability and migratory ability upon serum deprivation. Moreover, ST6Gal-i overexpression cells had strong resistance to paclitaxel, as demonstrated by low growth inhibition rate and cell apoptosis level. a mechanistic study showed that ST6Gal-i overexpression induced high α2,6-sialylation of FGFr1 and increased the expression of phospho-erK1/2 and phospho-focal adhesion kinase. Further study demonstrated that the FGFr1 inhibitor Pd173047 reduced cell viability and induced apoptosis; however, ST6Gal-i overexpression decreased the anticancer effect of Pd173047. in addition, ST6Gal-i overexpression attenuated the effect of adriamycin on cancer cells. collectively, these results suggested that FGFr1 sialylation plays an important role in cell migration and drug chemoresistance in ovarian cancer cells.
Comparison of the fluorescence intensity caused by the accumulation of PpIX in endometrial cancer xenografts in nude mice after low-dose 5-Aminolevulinic acid (ALA) injection combined with siRNA transfection was mediated by ultrasound microbubbles and polyethyleneimine (PEI) to explore the feasibility of the ultrasound microbubble technique as transfection agents. Knockdown of ferrochelatase (FECH) in human endometrial cancer xenografts in nude mice was performed by transfection with FECH-siRNA mediated by PEI and ultrasound microbubbles alone or in combination; then, low-dose ALA was injected. Subsequently, an in vivo animal imaging system was employed to detect the fluorescence intensity in xenografts. Red fluorescence was observed in xenografts given more than 6.25 mg kg À1 of ALA. When the dose of ALA was greater than 50 mg kg À1 , there was a significant difference in the fluorescence between tumor and other tissues. After the nude mice were transfected with siRNA and treated with low-dose ALA (1.0 mg kg À1 ), apparent PpIX fluorescence of the xenografts was observed, and the fluorescence intensity was PEI+ ultrasound microbubbles > PEI > ultrasound microbubbles. Ultrasound microbubbles in combination with PEI could generate a higher fluorescence intensity of PpIX than that obtained with ultrasound microbubbles or PEI alone, and ultrasound microbubbles could wholly or partially replace PEI under certain conditions. *Corresponding authors' e-mails: twxy163@163.com (Xiaoyu Wang); shaotsiang@ 163.com (Shaoqiang Lin) † Co-first authors.
Female pelvic floor dysfunctions are the most common diseases after pregnancy. Vaginal relaxation, stress urinary incontinence (SUI), and pelvic organ prolapse (POP), decreased sexual activity, and mental depression and other clinical symptoms, are the most normal results of childbirth which leading to cause various problems for the mother. We aim to explore the clinical situation and effect of postpartum pelvic floor rehabilitation nursing for patients with postpartum pelvic floor dysfunction. In this study, 100 patients with postpartum pelvic floor dysfunction randomly selected from January 2018 to December 2019. They were randomly divided into two groups for nursing care, including control group and observation group. The control group received routine care, and the observation group received postpartum pelvic floor rehabilitation care. We carry out comparison of the clinical nursing effects of the two groups. After analysis and statistics, the total clinical effectiveness (94.0%) and nursing satisfaction (92.0%) of the observation group were significantly higher than those of the control group (80.0%, 72.0%), and the differences between the groups were significant (P<0.05). In conclusion, implementing pelvic floor rehabilitation nursing for patients with postpartum pelvic floor dysfunction can better promote the rehabilitation of patients' pelvic floor function, improve clinical effects and nursing satisfaction.
Explore the application of case management in gestational diabetes mellitus (GDM). 164 patients diagnosed as GDM from February to November 2016 were set up as control group. 196 patients diagnosed as GDM from February to November of 2017 were divided into intervention group. In the control group, gynecology and obstetrics clinic doctors and nurses directly carried out routine health education, diet guidance and exercise advice. The intervention group adopted the case management model of gestational diabetes mellitus, including six major modules of case selection, overall service needs assessment, goal-oriented case management plan, follow-up management, contact and coordination to obtain services and evaluate case success, GDM women for blood glucose management, comparison of case management before and after GDM patients, the difference between the outcome of pregnancy. GDM case management model could effectively control the blood glucose of patients with GDM, reduce the readmission rate due to poor blood glucose control (P < 0.001), and significantly reduce the insulin used rate of patients with GDM (P < 0.019), but there was no significant difference between the two groups of the outcome of pregnancy (P > 0.05). Case management of gestational diabetes mellitus can effectively save medical costs and play an important role in the management of women with GDM.
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