BackgroundMonodehydroascorbate reductase (MDAR or MDHAR), which is responsible for growth, development and stress response in plants, is a key enzyme in the maintenance of the ascorbate acid (AsA) pool through the AsA–glutathione (AsA–GSH) cycle. High temperature affects a broad spectrum of cellular components and metabolism including AsA–GSH cycle in plants. In rice, however, the detailed roles of OsMDHAR4 in resistance against heat stress remains unclear.ResultsHere, we report that OsMDHAR4 protein was localized to the chloroplasts. OsMDHAR4 expression was detected in all tissues surveyed and peaked in leaf blade. OsMDHAR4 was responsive to multiple stresses and was relatively strongly induced by heat treatment. In comparison with wild type, the osmdhar4 mutant exhibited improved tolerance to heat stress, whereas OsMDHAR4 overexpression lines exhibited enhanced sensitivity to heat stress. Moreover, we found that suppression of OsMDHAR4 promoted stomatal closure and hydrogen peroxide accumulation, and overexpression of OsMDHAR4 increased stomatal opening and decreased hydrogen peroxide content in rice leaves.ConclusionsTaken together, these results indicated that OsMDHAR4 negatively regulates tolerance to heat stress by mediating H2O2-induced stomatal closure in rice.Electronic supplementary materialThe online version of this article (10.1186/s12284-018-0230-5) contains supplementary material, which is available to authorized users.
BackgroundDrought stress is one of the major abiotic stresses that restrict plant growth and development. 14–3-3 proteins have been validated to regulate many biological processes in plants. Previous research demonstrated that OsGF14b plays different roles in panicle and leaf blast resistance. In this study, we researched the function of OsGF14b in drought resistance in rice.FindingsHere, we report that OsGF14b was strongly induced by soil drought stress. In comparison with wild type (WT), the osgf14b mutant exhibited improved resistance to drought and osmotic stress by changing the content of stress-relevant parameters, complementation of the osgf14b mutant restored the drought sensitivity to WT levels, whereas the OsGF14b-overexpression lines exhibited enhanced sensitivity to drought and osmotic stress. The osgf14b mutant plants were hypersensitive to abscisic acid (ABA), while the OsGF14b-overexpression plants showed reduced sensitivity to ABA. Furthermore, mutation and overexpression of OsGF14b affected the expression of stress-related genes under normal growth conditions and/or drought stress conditions.ConclusionsWe have demonstrated that OsGF14b is involved in the drought resistance of rice plants, partially in an ABA-dependent manner.
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