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Rice is vulnerable to cold stress. Seedlings are very sensitive to cold stress and this harms global rice production. The effects of cold on chloroplast development are well known, but little is known about the underlying molecular mechanisms. Here, we isolated a temperature-sensitive virescent (tsv) mutant that is extremely sensitive to cold stress. It displayed defective chloroplasts, decreased chlorophyll and zero survivorship under cold stress. We isolated and identified TSV by map-based cloning and rescue experiments, combined with genetic, cytological and molecular biological analyses. We found that TSV, a putative plastidic oxidoreductase, is a new type of virescent protein. A mutation in tsv causes premature termination of the gene product. The activity of plastid-encoded RNA polymerase (PEP) and the expression of genes participating in chlorophyll synthesis were severely reduced in the tsv mutant under cold stress, but not at normal temperatures. TSV expression was induced by low temperatures. Strikingly, TSV interacted with OsTrxZ (a subunit of PEP in chloroplasts) and enhanced OsTrxZ stability under low temperatures. We demonstrated that TSV protects rice chloroplasts from cold stress by interacting with OsTrxZ. These results provide novel insights into ways in which rice chloroplast development and chlorophyll synthesis are protected by TSV under cold stress.

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Both SPOs and PSO showed similar effect in correcting the thoracolumbar kyphosis secondary to AS. However, patients treated with the SPOs technique showed higher risk in loss of correction in the instrumented region.

Objective: To compare two kinds of metal markers for preoperative localization of ground glass nodules (GGNs). Methods: We retrospectively investigated data from 198 cases of GGN localization and compared the success rate and complications of both approaches. Results: In the hook wire and coil groups, the success rates of CT-guided localization for GGNs were 99.2 and 98.7%, respectively (p = 1.000). The success rates of video-assisted thoracoscopic surgery in both groups were 100% without transthoracic surgery. The post-localization complication rates in the hook wire group and coil group were 36.9 and 32.9% (p = 0.568), and the postoperative complication rates in the hook wire and coil groups were 13.9 and 11.8%, respectively (p = 0.672). Conclusions: Preoperative localization of GGNs with both hook wire and coil methods proved to be useful and effective. Both methods have acceptable preoperative and postoperative complication rates, but the localization and operation times were shorter for the hook wire group than the coil group. Advances in knowledge: Most of previous articles studied a single preoperative localization method. Few studies have compared the preoperative and postoperative methods for metal markers. This paper compared two preoperative localization methods for GGNs to provide clinical guidance.

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In this paper, we propose a wirelessly powered Internet of Things (IoT) system based on the cell-free massive MIMO technology. In such a system, during the downlink phase, the sensors harvest radio-frequency (RF) energy emitted by the distributed access points (APs). During the uplink phase, sensors transmit data to the APs using the harvested energy. Collocated massive MIMO and small-cell IoT can be treated as special cases of cell-free IoT. We derive the tight closed-form lower bound on the amount of harvested energy, and the closedform expression of SINR as the metrics of power transfer and data transmission, respectively. To improve the energy efficiency, we jointly optimize the uplink and downlink power control coefficients to minimize the total transmit energy consumption while meeting the target SINRs. Extended simulation results show that cell-free IoT outperforms collocated massive MIMO and small-cell IoT both in terms of the per user throughput for uplink, and the amount of energy harvested for downlink. Moreover, significant gains can be achieved by the proposed joint power control in terms of both per user throughput and energy consumption.Index Terms-Cell-free massive MIMO, Internet-of-things, power control, wireless power transfer.