There has been growing interest in coherent optical orthogonal frequencydivision multiplexing (CO-OFDM). We aim to present the case that the time for CO-OFDM has come, in terms of the demand from today's ever-advancing optical networks and the availability of its underlying technologies. We first lay out the signal processing aspect for CO-OFDM and then show a 2 ϫ 2 multiple-input-multiple-output (MIMO) OFDM representation for the singlemode fiber optical channel. Through numerical simulation and experimental demonstration, we further present and discuss various MIMO-OFDM systems focusing on their polarization-mode dispersion resilience. Among those systems, the 2 ϫ 1 MIMO-OFDM configuration incorporating polarization-time coding shows promise for optical access networks and broadcast networks. Finally, another class of the frequency-domain equalization techniques, namely, incoherent or coherent optical single-carrier frequency-domain equalization (CO-SCFDE) is discussed and experimentally demonstrated.

Rationale Anti-angiogenesis therapies such as bevacizumab, the monoclonal antibody to vascular endothelial growth factor (VEGF), have been used against ovarian cancer, but transient and low peritoneal drug levels are likely a factor in treatment failure. We hypothesized that a single administration of adeno-associated virus (AAV)-mediated intraperitoneal expression of bevacizumab would direct persistent expression and suppress growth and metastasis of ovarian cancer. Methods AAVrh10.BevMab, a rhesus serotype 10 adeno-associated viral vector coding for bevacizumab, was evaluated for the capacity of a single intraperitoneal administration to persistently suppress peritoneal tumor growth in an intraperitoneal model of ovarian carcinomatosis with human ovarian cancer cells in nude immunodeficient mice. Results The data demonstrates that AAVrh10.BevMab mediates persistent and high levels of bevacizumab in the peritoneal cavity following a single intraperitoneal administration in mice. In AAVrh10.BevMab treated A2780 human ovarian cancer-bearing mice, tumor growth was significantly suppressed (p<0.05) and the area of blood vessels in the tumor were decreased (p<0.04). Survival of mice with A2780 xenografts or SK-OV3 xenografts was greatly prolonged in the presence of AAVrh10.BevMab (p<0.001). Administration of AAVrh10.BevMab 4 days after A2780-luciferase cell implantation reduced tumor growth (p<0.01) and increased mouse survival (p<0.0001). Combination of AAVrh10.BevMab with cytotoxic reagents paclitaxel or topotecan proved to be more effective in increasing survival than treatment with cytotoxic reagent alone. Conclusion A single administration of AAVrh10.BevMab provides sustained and high local expression of bevacizumab in the peritoneal cavity, and significantly suppresses peritoneal carcinomatosis and increases survival in an ovarian cancer murine model.

Time to treatment of seizures is critical to efficacy. We performed a quality initiative and evaluated time to treatment of inpatient seizure emergencies with first- and second-line medicines before and after implementation of a computerized, standard treatment protocol. Data from 125 patients revealed that 179 seizure episodes required first-line antiepileptic drugs, and the mean time to treatment was 7.72 minutes. In 87 episodes, patients (49%) received the drugs within 5 minutes. Forty-six episodes required second-line drugs. In 17 (37%), patients received them within 30 minutes (mean 49.48 minutes). After implementation of the protocol, the mean time to treatment with first-line drugs was 3.74 minutes, a reduction of >50% (P < .0001). The mean time to treatment with second-line drugs was 25.05 minutes, a reduction of ∼50% (P < .0001). This effective model for reducing the time to treatment of seizure emergencies may be useful to similar institutions.

Abstract-There are three major advantages for coherent optical orthogonal frequency-division multiplexing (CO-OFDM) transmission using digital signal processing. First, coherent detection is realized by digital phase estimation without the need for optical phase-locked loop. Second, OFDM modulation and demodulation are realized by the well-established computation-efficient fast Fourier transform (FFT) and inverse FFT. Third, adaptive data rates can be supported as different quadrature amplitude modulation (QAM) constellations are software-defined, without any hardware change in transmitter and receiver. However, it is well-known that coherent detection, OFDM, and QAM are all susceptible to phase noise. In this paper, theoretical, numerical, and experimental investigations are carried out for phase noise effects on high spectral efficiency CO-OFDM transmission. A transmission model in the presence of phase noise is presented. By using simulation, the bit error rate floors from finite laser linewidth are presented for CO-OFDM systems with high-order QAM constellations. In the experiments, the phase noise effects from both laser linewidth and nonlinear fiber transmission are investigated. The fiber nonlinearity mitigation based on receiver digital signal processing is also discussed.